Tubulin Beta 4A antibody | 8E3

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Anti Tubulin Beta 4A Antibody, clone 8E3 (PrecisionAb™ Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with tubulin beta 4A antibody followed by detection with HRP conjugated Goat anti Mouse IgG antibody (1/10,000, STAR207P) and visualized on the ChemiDoc™ MP with 12 second exposure. Arrow points to tubulin beta 4A (molecular weight 52 kDa).

Mouse anti Tubulin Beta 4A

Mouse anti-human tubulin beta 4A antibody recognizes tubulin beta-4A chain, also known as dystonia 4, torsion (autosomal dominant) or tubulin beta-4A chain. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type: PrecisionAb™ Monoclonal
Clone: 8E3
Isotype: IgG2a
Format: Purified

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
VMA00462 Datasheet	WB MSDS	100 µl	Log in		Get Quote

Summary
Secondary Antibodies

Mouse anti Human tubulin beta 4A antibody recognizes Tubulin beta 4A, also known as dystonia 4, torsion (autosomal dominant), TUBB4, tubulin, beta 4 or tubulin beta 5.

Mouse anti Human Tubulin Beta 4A antibody detects a band of 52 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species

Human

Western Blotting

Anti tubulin beta 4a detects a band of approximately 52 kDa in Jurkat cell lysate

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein A from ascites

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol

Immunogen

Full length recombinant protein of human tubulin beta 4A produced in HEK293T cell

Instructions For Use

Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Guarantee: As supplied, 12 months from date of despatch.

More Information

UniProt: P04350 Related reagents
Entrez Gene: TUBB4 Related reagents
GO Terms: GO:0000086 G2/M transition of mitotic cell cycle; GO:0005515 protein binding; GO:0003924 GTPase activity; GO:0005829 cytosol; GO:0005198 structural molecule activity; GO:0005525 GTP binding; GO:0005874 microtubule; GO:0007018 microtubule-based movement; GO:0051084 'de novo' posttranslational protein folding; GO:0051258 protein polymerization
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of Tubulin Beta 4A antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in		Get Quote

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