Tubulin Beta 4A antibody | 8E3
Mouse anti Tubulin Beta 4A
Mouse anti-human tubulin beta 4A antibody recognizes tubulin beta-4A chain, also known as dystonia 4, torsion (autosomal dominant) or tubulin beta-4A chain. This antibody has been extensively validated for western blotting using whole cell lysates.
Tubulin Beta 4A Antibody with Control Lysate
Mouse anti-human tubulin beta 4A antibody recognizes tubulin beta-4A chain, also known as dystonia 4, torsion (autosomal dominant) or tubulin beta-4A chain. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Monoclonal
- Clone
- 8E3
- Isotype
- IgG2a
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00462 | WB | 100 µl | ||||
| VMA00462KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human tubulin beta 4A antibody recognizes Tubulin beta 4A, also known as dystonia 4, torsion (autosomal dominant), TUBB4, tubulin, beta 4 or tubulin beta 5.
Mouse anti Human Tubulin Beta 4A antibody detects a band of 52 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Mouse anti Human Tubulin Beta 4A antibody detects a band of 52 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti tubulin beta 4a detects a band of approximately 52 kDa in Jurkat cell lysate
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from ascites
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatography on Protein A from ascites
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- Full length recombinant protein of human tubulin beta 4A produced in HEK293T cell
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg Jurkat lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P04350 Related reagents
- Entrez Gene
- TUBB4 Related reagents
- GO Terms
- GO:0000086 G2/M transition of mitotic cell cycle
- GO:0005515 protein binding
- GO:0003924 GTPase activity
- GO:0005829 cytosol
- GO:0005198 structural molecule activity
- GO:0005525 GTP binding
- GO:0005874 microtubule
- GO:0007018 microtubule-based movement
- GO:0051084 'de novo' posttranslational protein folding
- GO:0051258 protein polymerization
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of Tubulin Beta 4A antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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