Tubulin Alpha antibody

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Rat anti Tubulin Alpha

Tubulin alpha antibody recognizes alpha tubulin, a 451 amino acid protein and major constituent of microtubules. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG2a
Format
Purified
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00051 WB 100 µl
Rat anti Human Tubulin alpha antibody recognizes alpha tubulin, a 451 amino acid protein and major constituent of microtubules. Rat anti Human tubulin alpha antibody binds to a C-terminal epitope located between residues 414 and 422. Alpha tubulin is highly conserved between species and is expressed at high levels in most cells under a variety of conditions rendering it a suitable target as a loading control for western blotting.

Rat anti Human tubulin alpha antibody recognizes alpha tubulin as a single band of ~52 kDa in multiple cell lysates from multiple species by western blotting under reducing conditions.

Product Details

Target Species
Human
Western Blotting
Anti tubulin alpha antibody detects a band of approximately 52 kDa in Jurkat cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rat monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Yeast tubulin
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Tubulin Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
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