Tubulin Alpha antibody

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Rat anti Tubulin Alpha

Product Type
PrecisionAb Monoclonal
Isotype
IgG2a
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00051
Datasheet
WB
MSDS
100 µl loader

Rat anti Human Tubulin alpha antibody recognizes alpha tubulin, a 451 amino acid protein and major constituent of microtubules. Rat anti Human tubulin alpha antibody binds to a C-terminal epitope located between residues 414 and 422. Alpha tubulin is highly conserved between species and is expressed at high levels in most cells under a variety of conditions rendering it a suitable target as a loading control for western blotting.

Rat anti Human tubulin alpha antibody recognizes alpha tubulin as a single band of ~52 kDa in multiple cell lysates from multiple species by western blotting under reducing conditions.

Product Details

Target Species
Human
Western Blotting
Anti tubulin alpha antibody detects a band of approximately 52 kDa in Jurkat cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rat monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Yeast tubulin

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Tubulin Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader

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