TUBG1 antibody | CD01/3H7
Mouse anti TUBG1 antibody recognizes tubulin gamma-1 chain, also known as TBG1 and GCP-1.
The gamma tubulin TUBG1 is a major part of the centrosome in mammalian cells, and is important for microtubule formation and cell cycle progression (Brock et al. 2018). The other gene that encodes gamma tubulin in mammals, TUBG2, is only expressed in preimplantation embryos and the brain, whereas TUBG1 appears to be ubiquitously expressed in mammalian cells (Ohashi et al. 2016). TUBG1 is also abundantly expressed in the cerebral cortex, cerebellum, thalamus and hippocampus of the developing and mature human brain (Draberova et al. 2017). Variants in the gene encoding TUBG1 can cause tubulinopathies, characterised by a range of cortical malformations, intellectual disability and epilepsy (Ivanova et al. 2019).
The gamma tubulin TUBG1 is a major part of the centrosome in mammalian cells, and is important for microtubule formation and cell cycle progression (Brock et al. 2018). The other gene that encodes gamma tubulin in mammals, TUBG2, is only expressed in preimplantation embryos and the brain, whereas TUBG1 appears to be ubiquitously expressed in mammalian cells (Ohashi et al. 2016). TUBG1 is also abundantly expressed in the cerebral cortex, cerebellum, thalamus and hippocampus of the developing and mature human brain (Draberova et al. 2017). Variants in the gene encoding TUBG1 can cause tubulinopathies, characterised by a range of cortical malformations, intellectual disability and epilepsy (Ivanova et al. 2019).
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti TUBG1 detects a band of approximately 46 kDa in Jurkat cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - Liquid
- Preparation
- Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
- Immunogen
- E. coli-derived recombinant protein of amino acids 1-451 of human TUBG1
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P23258
- Entrez Gene
- TUBG1
- GO Terms
- GO:0000086 G2/M transition of mitotic cell cycle
- GO:0005515 protein binding
- GO:0000212 meiotic spindle organization
- GO:0000794 condensed nuclear chromosome
- GO:0003924 GTPase activity
- GO:0000930 gamma-tubulin complex
- GO:0005829 cytosol
- GO:0005200 structural constituent of cytoskeleton
- GO:0005525 GTP binding
- GO:0005827 polar microtubule
- GO:0051258 protein polymerization
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of TUBG1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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