TSC1 antibody | OTI3A2

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Anti TSC1 Antibody, clone OTI3A2 (PrecisionAb™ Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with TSC1 antibody (**VMA00181**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc™ MP with 105 second exposure. Arrow points to TSC1 (molecular weight 150 kDa).

Mouse anti TSC1

TSC1 antibody recognizes hamartin, also known as tuberous sclerosis 1. This antibody has been extensively validated for western blotting using whole cell lysates.

TSC1 Antibody with Control Lysate

TSC1 antibody recognizes hamartin, also known as tuberous sclerosis 1. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type: PrecisionAb™ Monoclonal
Clone: OTI3A2
Isotype: IgG2a
Format: Purified

Product Code	Applications	Pack Size
VMA00181	WB	100 µl
VMA00181KT	WB	2 Westerns
With Lysate		400µg

Summary
Secondary Antibodies

Mouse anti Human TSC1 antibody recognizes TSC1, also known as hamartin and tuberous sclerosis 1 protein.

TSC1 encodes a growth inhibitory protein thought to play a role in the stabilization of tuberin. Mutations in TSC1 have been associated with tuberous sclerosis. Alternative splicing results in multiple transcript variants (provided by RefSeq, Jun 2009).

Mouse anti Human TSC1 antibody detects a band of 150 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species: Human
Western Blotting: Anti TSC1 detects a band of approximately 150 kDa in Jurkat cell lysates.
Product Form: Purified IgG - liquid
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography from ascites.
Preparation: 20μl Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Immunogen: Human recombinant protein fragment corresponding to amino acids 836-1164 of human TSC1 (NP_000359) produced in E. coli
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition: 400μg Jurkat lysate lyophilized in RIPA buffer.
Lysate Reconstitution: - If using DDT reconstitute the lyophilized lysate with 190μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Storage: Antibody: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life: As supplied, 12 months from date of despatch.
Shelf Life: As supplied, 12 months from date of despatch.

More Information

UniProt: Q92574 Related reagents
Entrez Gene: TSC1 Related reagents
GO Terms: GO:0051087 chaperone binding; GO:0016020 membrane; GO:0005938 cell cortex; GO:0006407 rRNA export from nucleus; GO:0007050 cell cycle arrest; GO:0007160 cell-matrix adhesion; GO:0008285 negative regulation of cell proliferation; GO:0008286 insulin receptor signaling pathway; GO:0017148 negative regulation of translation; GO:0030027 lamellipodium; GO:0031397 negative regulation of protein ubiquitination; GO:0032007 negative regulation of TOR signaling cascade; GO:0032862 activation of Rho GTPase activity; GO:0033596 TSC1-TSC2 complex; GO:0043666 regulation of phosphoprotein phosphatase activity; GO:0047485 protein N-terminus binding; GO:0051492 regulation of stress fiber assembly; GO:0051894 positive regulation of focal adhesion assembly
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of TSC1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB

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