TSC1 antibody | OTI3A2

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Mouse anti TSC1

TSC1 antibody recognizes hamartin, also known as tuberous sclerosis 1. This antibody has been extensively validated for western blotting using whole cell lysates.

TSC1 Antibody with Control Lysate

TSC1 antibody recognizes hamartin, also known as tuberous sclerosis 1. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Clone
OTI3A2
Isotype
IgG2a
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00181 WB 100 µl
VMA00181KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human TSC1 antibody recognizes TSC1, also known as hamartin and tuberous sclerosis 1 protein.

TSC1 encodes a growth inhibitory protein thought to play a role in the stabilization of tuberin. Mutations in TSC1 have been associated with tuberous sclerosis. Alternative splicing results in multiple transcript variants (provided by RefSeq, Jun 2009).

Mouse anti Human TSC1 antibody detects a band of 150 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti TSC1 detects a band of approximately 150 kDa in Jurkat cell lysates.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites.
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Human recombinant protein fragment corresponding to amino acids 836-1164 of human TSC1 (NP_000359) produced in E. coli
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg Jurkat lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
Q92574 Related reagents
Entrez Gene
TSC1 Related reagents
GO Terms
GO:0051087 chaperone binding
GO:0016020 membrane
GO:0005938 cell cortex
GO:0006407 rRNA export from nucleus
GO:0007050 cell cycle arrest
GO:0007160 cell-matrix adhesion
GO:0008285 negative regulation of cell proliferation
GO:0008286 insulin receptor signaling pathway
GO:0017148 negative regulation of translation
GO:0030027 lamellipodium
GO:0031397 negative regulation of protein ubiquitination
GO:0032007 negative regulation of TOR signaling cascade
GO:0032862 activation of Rho GTPase activity
GO:0033596 TSC1-TSC2 complex
GO:0043666 regulation of phosphoprotein phosphatase activity
GO:0047485 protein N-terminus binding
GO:0051492 regulation of stress fiber assembly
GO:0051894 positive regulation of focal adhesion assembly
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of TSC1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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