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TSC1 antibody | OTI3A2

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Western Blot Validated
Anti TSC1 Antibody, clone OTI3A2 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with TSC1 antibody (VMA00181) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc™ MP with 105 second exposure. Arrow points to TSC1 (molecular weight 150 kDa).

Mouse anti TSC1

Product Type
PrecisionAb Monoclonal
Clone
OTI3A2
Isotype
IgG2a
Format
Purified
PrecisionAb Antibodies
Product Code Applications Pack Size List Price Quantity
VMA00181
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human TSC1 antibody recognizes TSC1, also known as hamartin and tuberous sclerosis 1 protein.

TSC1 encodes a growth inhibitory protein thought to play a role in the stabilization of tuberin. Mutations in TSC1 have been associated with tuberous sclerosis. Alternative splicing results in multiple transcript variants (provided by RefSeq, Jun 2009).

Mouse anti Human TSC1 antibody detects a band of 150 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti TSC1 detects a band of approximately 150 kDa in Jurkat cell lysates.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Human recombinant protein fragment corresponding to amino acids 836-1164 of human TSC1 (NP_000359) produced in E. coli

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
Q92574
Entrez Gene
TSC1
GO Terms
GO:0051087 chaperone binding
GO:0016020 membrane
GO:0005938 cell cortex
GO:0006407 rRNA export from nucleus
GO:0007050 cell cycle arrest
GO:0007160 cell-matrix adhesion
GO:0008285 negative regulation of cell proliferation
GO:0008286 insulin receptor signaling pathway
GO:0017148 negative regulation of translation
GO:0030027 lamellipodium
GO:0031397 negative regulation of protein ubiquitination
GO:0032007 negative regulation of TOR signaling cascade
GO:0032862 activation of Rho GTPase activity
GO:0033596 TSC1-TSC2 complex
GO:0043666 regulation of phosphoprotein phosphatase activity
GO:0047485 protein N-terminus binding
GO:0051492 regulation of stress fiber assembly
GO:0051894 positive regulation of focal adhesion assembly
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of TSC1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

A Precise Solution to Reproducible Western Blots - PrecisionAb Antibodies
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