Troponin I (Cardiac) antibody
Mouse anti Human troponin I (cardiac) antibody recognizes the troponin I (TnI) protein expressed in the heart tissue also known as troponin I type 3 (cardiac). Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. Encoded by the TNN13 gene, TnI-cardiac is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM) (provided by RefSeq, Jul 2008).
Mouse anti Human troponin I (cardiac) antibody detects a band of 24 kDa. The antibody has been validated for western blotting using human heart lysate.
Mouse anti Human troponin I (cardiac) antibody detects a band of 24 kDa. The antibody has been validated for western blotting using human heart lysate.
Product Details
- Target Species
- Human
- Western Blotting
- Anti troponin (cardiac) detects a band of approximately 24 kDa in human heart tissue
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Native troponin I protein purified from human cardiac tissue
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P19429
- Entrez Gene
- TNNI3
- GO Terms
- GO:0001570 vasculogenesis
- GO:0001980 regulation of systemic arterial blood pressure by ischemic conditions
- GO:0003779 actin binding
- GO:0005829 cytosol
- GO:0005861 troponin complex
- GO:0006874 cellular calcium ion homeostasis
- GO:0019855 calcium channel inhibitor activity
- GO:0019901 protein kinase binding
- GO:0019904 protein domain specific binding
- GO:0030049 muscle filament sliding
- GO:0030172 troponin C binding
- GO:0031014 troponin T binding
- GO:0032780 negative regulation of ATPase activity
- GO:0048306 calcium-dependent protein binding
- GO:0055010 ventricular cardiac muscle tissue morphogenesis
- GO:0060048 cardiac muscle contraction
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of Troponin I (Cardiac) antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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