Troponin I (Cardiac) antibody

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Mouse anti Troponin I (Cardiac)

Product Type
PrecisionAb Monoclonal
Isotype
IgG2a
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00419
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human troponin I (cardiac) antibody recognizes the troponin I (TnI) protein expressed in the heart tissue also known as troponin I type 3 (cardiac). Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. Encoded by the TNN13 gene, TnI-cardiac is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM) (provided by RefSeq, Jul 2008).

Mouse anti Human troponin I (cardiac) antibody detects a band of 24 kDa. The antibody has been validated for western blotting using human heart lysate.

Product Details

Target Species
Human
Western Blotting
Anti troponin (cardiac) detects a band of approximately 24 kDa in human heart tissue
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Native troponin I protein purified from human cardiac tissue

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
P19429 Related reagents
Entrez Gene
TNNI3 Related reagents
GO Terms
GO:0001570 vasculogenesis
GO:0001980 regulation of systemic arterial blood pressure by ischemic conditions
GO:0003779 actin binding
GO:0005829 cytosol
GO:0005861 troponin complex
GO:0006874 cellular calcium ion homeostasis
GO:0019855 calcium channel inhibitor activity
GO:0019901 protein kinase binding
GO:0019904 protein domain specific binding
GO:0030049 muscle filament sliding
GO:0030172 troponin C binding
GO:0031014 troponin T binding
GO:0032780 negative regulation of ATPase activity
GO:0048306 calcium-dependent protein binding
GO:0055010 ventricular cardiac muscle tissue morphogenesis
GO:0060048 cardiac muscle contraction
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Troponin I (Cardiac) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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