TRIM24 antibody | OTI2D9
Mouse anti TRIM24
Mouse anti human TRIM24 antibody recognizes transcription intermediary factor 1-alpha, also known as RING finger protein 82, TIF1-alpha, transcriptional intermediary factor 1 or tripartite motif-containing 24. This antibody has been validated for western blotting using whole cell lysates.
TRIM24 Antibody with Control Lysate
Mouse anti human TRIM24 antibody recognizes transcription intermediary factor 1-alpha, also known as RING finger protein 82, TIF1-alpha, transcriptional intermediary factor 1 or tripartite motif-containing 24. This antibody has been extensively validated for western blotting.
- Product Type
- PrecisionAb™ Monoclonal
- Clone
- OTI2D9
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00396 | WB | 100 µl | ||||
| VMA00396KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human TRIM24 antibody recognizes E3 ubiquitin-protein ligase TRIM24, also known as RING finger protein 82, TIF1-alpha, transcriptional intermediary factor 1 and tripartite motif-containing 24.
Encoded by the TRIM24 gene, E3 ubiquitin-protein ligase TRIM24 mediates transcriptional control by interaction with the activation function 2 (AF2) region of several nuclear receptors, including the estrogen, retinoic acid, and vitamin D3 receptors. The protein localizes to nuclear bodies and is thought to associate with chromatin and heterochromatin-associated factors. The protein is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains - a RING, a B-box type 1 and a B-box type 2 - and a coiled-coil region. Two alternatively spliced transcript variants encoding different isoforms have been described for TRIM24 (provided by RefSeq, Jul 2008).
Mouse anti Human TRIM24 antibody detects a band of 130 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Encoded by the TRIM24 gene, E3 ubiquitin-protein ligase TRIM24 mediates transcriptional control by interaction with the activation function 2 (AF2) region of several nuclear receptors, including the estrogen, retinoic acid, and vitamin D3 receptors. The protein localizes to nuclear bodies and is thought to associate with chromatin and heterochromatin-associated factors. The protein is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains - a RING, a B-box type 1 and a B-box type 2 - and a coiled-coil region. Two alternatively spliced transcript variants encoding different isoforms have been described for TRIM24 (provided by RefSeq, Jul 2008).
Mouse anti Human TRIM24 antibody detects a band of 130 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti TRIM24 detects a band of approximately 130 kDa in HepG2 cell lysates
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography from ascites
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatography from ascites
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- Recombinant protein fragment corresponding to aa 706-1016 of human TRIM24 (NP_003843) produced in E.coli
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HepG2 lysate lyophilized in RIPA buffer
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Guarantee
- As supplied, 12 months from date of despatch.
- Guarantee
- As supplied, 12 months from date of despatch
More Information
- UniProt
- O15164 Related reagents
- Entrez Gene
- TRIM24 Related reagents
- GO Terms
- GO:0002039 p53 binding
- GO:0003682 chromatin binding
- GO:0003713 transcription coactivator activity
- GO:0004842 ubiquitin-protein ligase activity
- GO:0006366 transcription from RNA polymerase II promoter
- GO:0008270 zinc ion binding
- GO:0042981 regulation of apoptosis
- GO:0030163 protein catabolic process
- GO:0031647 regulation of protein stability
- GO:0034056 estrogen response element binding
- GO:0070577 histone acetyl-lysine binding
- GO:0071391 cellular response to estrogen stimulus
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of TRIM24 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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