TRF1 antibody

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Mouse anti TRF1

TRF1 antibody recognizes telomeric repeat-binding factor 1, also known as NIMA-interacting protein 2, TTAGGG repeat-binding factor 1 or telomeric protein Pin2/TRF1. The antibody has been extensively validated for western blotting using whole cell lysates.

TRF1 Antibody with Control Lysate

TRF1 antibody recognizes telomeric repeat-binding factor 1, also known as NIMA-interacting protein 2, TTAGGG repeat-binding factor 1 or telomeric protein Pin2/TRF1. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG1
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00032 WB 100 µl
VMA00032KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human TRF1 antibody recognizes human telomeric repeat-binding factor 1, also known as TTAGGG repeat binding factor 1 (TRF1) or NIMA-interacting protein 2. TRF1 is a ubiquitously expressed 439 amino acid DNA binding protein and negative regulator of telomere length containing a single Myb domain. Along with tankyrase-1, TIN2, and PINX1, TRF1 binds the double-stranded TTAGGG repeat of telomeres and regulates telomere length. Protection of telomeres-1 (POT1) is a single-stranded telomeric DNA-binding protein which controls the elongation of telomeres by telomerase. TRF1 mediates the loading of POT1 onto single stranded DNA, conveying information about telomere length to the telomere terminus, where telomerase is regulated. If the telomere is long enough, telomerase is inhibited.

Additionally, in conjunction with POT1 and TTAGGG repeat binding factor 2 (TRF2), TRF1 recruits factors to the telomere, inducing the formation of the protective t-loop. TRF1 along with TRF2, POT1, TIN2, TPP1 and Rap1 form the shelterin complex which distinguishes telomeres from sites of DNA damage (Wellinger 2009). TRF1 is thought to play a role in leukaemia (Shi et al. 2006).

Mouse anti Human TRF1 antibody recognizes human TRF1 as a single band of ~60 kDa in multiple human cell line lysates by western blotting under reducing conditions.

Product Details

Target Species
Human
Western Blotting
Anti TRF1 antibody detects a band of approximately 60 kDa in K562 cell lysates.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant TRF1
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg K562 lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P54274 Related reagents
Entrez Gene
TERF1 Related reagents
GO Terms
GO:0000086 G2/M transition of mitotic cell cycle
GO:0000781 chromosome, telomeric region
GO:0000783 nuclear telomere cap complex
GO:0001309 age-dependent telomere shortening
GO:0003691 double-stranded telomeric DNA binding
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005654 nucleoplasm
GO:0005819 spindle
GO:0006917 induction of apoptosis
GO:0006919 activation of caspase activity
GO:0042162 telomeric DNA binding
GO:0007004 telomere maintenance via telomerase
GO:0007067 mitosis
GO:0007094 mitotic cell cycle spindle assembly checkpoint
GO:0008017 microtubule binding
GO:0008301 DNA bending activity
GO:0042802 identical protein binding
GO:0008656 caspase activator activity
GO:0010521 telomerase inhibitor activity
GO:0010834 telomere maintenance via telomere shortening
GO:0031116 positive regulation of microtubule polymerization
GO:0032211 negative regulation of telomere maintenance via telomerase
GO:0032214 negative regulation of telomere maintenance via semi-conservative replication
GO:0042803 protein homodimerization activity
GO:0051260 protein homooligomerization
GO:0045449 regulation of transcription
GO:0045840 positive regulation of mitosis
GO:0045931 positive regulation of mitotic cell cycle
GO:0046982 protein heterodimerization activity
GO:0051301 cell division
GO:0051974 negative regulation of telomerase activity
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of TRF1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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