TRF1 antibody

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Mouse anti TRF1

TRF1 antibody recognizes telomeric repeat-binding factor 1, also known as NIMA-interacting protein 2, TTAGGG repeat-binding factor 1 or telomeric protein Pin2/TRF1. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Type: PrecisionAb™ Monoclonal
Isotype: IgG1
Format: Purified

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
VMA00032 Datasheet	WB MSDS	100 µl	Log in		Get Quote

Summary
Secondary Antibodies

Mouse anti Human TRF1 antibody recognizes human telomeric repeat-binding factor 1, also known as TTAGGG repeat binding factor 1 (TRF1) or NIMA-interacting protein 2. TRF1 is a ubiquitously expressed 439 amino acid DNA binding protein and negative regulator of telomere length containing a single Myb domain. Along with tankyrase-1, TIN2, and PINX1, TRF1 binds the double-stranded TTAGGG repeat of telomeres and regulates telomere length. Protection of telomeres-1 (POT1) is a single-stranded telomeric DNA-binding protein which controls the elongation of telomeres by telomerase. TRF1 mediates the loading of POT1 onto single stranded DNA, conveying information about telomere length to the telomere terminus, where telomerase is regulated. If the telomere is long enough, telomerase is inhibited.

Additionally, in conjunction with POT1 and TTAGGG repeat binding factor 2 (TRF2), TRF1 recruits factors to the telomere, inducing the formation of the protective t-loop. TRF1 along with TRF2, POT1, TIN2, TPP1 and Rap1 form the shelterin complex which distinguishes telomeres from sites of DNA damage (Wellinger 2009). TRF1 is thought to play a role in leukaemia (Shi et al. 2006).

Mouse anti Human TRF1 antibody recognizes human TRF1 as a single band of ~60 kDa in multiple human cell line lysates by western blotting under reducing conditions.

Product Details

Target Species: Human
Western Blotting: Anti TRF1 antibody detects a band of approximately 60 kDa in K562 cell lysates.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Immunogen: Recombinant TRF1
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Guarantee: As supplied, 12 months from date of despatch.

More Information

UniProt: P54274 Related reagents
Entrez Gene: TERF1 Related reagents
GO Terms: GO:0000086 G2/M transition of mitotic cell cycle; GO:0000781 chromosome, telomeric region; GO:0000783 nuclear telomere cap complex; GO:0001309 age-dependent telomere shortening; GO:0003691 double-stranded telomeric DNA binding; GO:0005634 nucleus; GO:0005737 cytoplasm; GO:0005654 nucleoplasm; GO:0005819 spindle; GO:0006917 induction of apoptosis; GO:0006919 activation of caspase activity; GO:0042162 telomeric DNA binding; GO:0007004 telomere maintenance via telomerase; GO:0007067 mitosis; GO:0007094 mitotic cell cycle spindle assembly checkpoint; GO:0008017 microtubule binding; GO:0008301 DNA bending activity; GO:0042802 identical protein binding; GO:0008656 caspase activator activity; GO:0010521 telomerase inhibitor activity; GO:0010834 telomere maintenance via telomere shortening; GO:0031116 positive regulation of microtubule polymerization; GO:0032211 negative regulation of telomere maintenance via telomerase; GO:0032214 negative regulation of telomere maintenance via semi-conservative replication; GO:0042803 protein homodimerization activity; GO:0051260 protein homooligomerization; GO:0045449 regulation of transcription; GO:0045840 positive regulation of mitosis; GO:0045931 positive regulation of mitotic cell cycle; GO:0046982 protein heterodimerization activity; GO:0051301 cell division; GO:0051974 negative regulation of telomerase activity
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of TRF1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

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