TRF1 antibody
Mouse anti TRF1
TRF1 antibody recognizes telomeric repeat-binding factor 1, also known as NIMA-interacting protein 2, TTAGGG repeat-binding factor 1 or telomeric protein Pin2/TRF1. The antibody has been extensively validated for western blotting using whole cell lysates.
TRF1 Antibody with Control Lysate
TRF1 antibody recognizes telomeric repeat-binding factor 1, also known as NIMA-interacting protein 2, TTAGGG repeat-binding factor 1 or telomeric protein Pin2/TRF1. The antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Monoclonal
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00032 | WB | 100 µl | ||||
| VMA00032KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human TRF1 antibody recognizes human telomeric repeat-binding factor 1, also known as TTAGGG repeat binding factor 1 (TRF1) or NIMA-interacting protein 2. TRF1 is a ubiquitously expressed 439 amino acid DNA binding protein and negative regulator of telomere length containing a single Myb domain. Along with tankyrase-1, TIN2, and PINX1, TRF1 binds the double-stranded TTAGGG repeat of telomeres and regulates telomere length. Protection of telomeres-1 (POT1) is a single-stranded telomeric DNA-binding protein which controls the elongation of telomeres by telomerase. TRF1 mediates the loading of POT1 onto single stranded DNA, conveying information about telomere length to the telomere terminus, where telomerase is regulated. If the telomere is long enough, telomerase is inhibited.
Additionally, in conjunction with POT1 and TTAGGG repeat binding factor 2 (TRF2), TRF1 recruits factors to the telomere, inducing the formation of the protective t-loop. TRF1 along with TRF2, POT1, TIN2, TPP1 and Rap1 form the shelterin complex which distinguishes telomeres from sites of DNA damage (Wellinger 2009). TRF1 is thought to play a role in leukaemia (Shi et al. 2006).
Mouse anti Human TRF1 antibody recognizes human TRF1 as a single band of ~60 kDa in multiple human cell line lysates by western blotting under reducing conditions.
Additionally, in conjunction with POT1 and TTAGGG repeat binding factor 2 (TRF2), TRF1 recruits factors to the telomere, inducing the formation of the protective t-loop. TRF1 along with TRF2, POT1, TIN2, TPP1 and Rap1 form the shelterin complex which distinguishes telomeres from sites of DNA damage (Wellinger 2009). TRF1 is thought to play a role in leukaemia (Shi et al. 2006).
Mouse anti Human TRF1 antibody recognizes human TRF1 as a single band of ~60 kDa in multiple human cell line lysates by western blotting under reducing conditions.
Product Details
- Target Species
- Human
- Western Blotting
- Anti TRF1 antibody detects a band of approximately 60 kDa in K562 cell lysates.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Recombinant TRF1
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg K562 lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P54274 Related reagents
- Entrez Gene
- TERF1 Related reagents
- GO Terms
- GO:0000086 G2/M transition of mitotic cell cycle
- GO:0000781 chromosome, telomeric region
- GO:0000783 nuclear telomere cap complex
- GO:0001309 age-dependent telomere shortening
- GO:0003691 double-stranded telomeric DNA binding
- GO:0005634 nucleus
- GO:0005737 cytoplasm
- GO:0005654 nucleoplasm
- GO:0005819 spindle
- GO:0006917 induction of apoptosis
- GO:0006919 activation of caspase activity
- GO:0042162 telomeric DNA binding
- GO:0007004 telomere maintenance via telomerase
- GO:0007067 mitosis
- GO:0007094 mitotic cell cycle spindle assembly checkpoint
- GO:0008017 microtubule binding
- GO:0008301 DNA bending activity
- GO:0042802 identical protein binding
- GO:0008656 caspase activator activity
- GO:0010521 telomerase inhibitor activity
- GO:0010834 telomere maintenance via telomere shortening
- GO:0031116 positive regulation of microtubule polymerization
- GO:0032211 negative regulation of telomere maintenance via telomerase
- GO:0032214 negative regulation of telomere maintenance via semi-conservative replication
- GO:0042803 protein homodimerization activity
- GO:0051260 protein homooligomerization
- GO:0045449 regulation of transcription
- GO:0045840 positive regulation of mitosis
- GO:0045931 positive regulation of mitotic cell cycle
- GO:0046982 protein heterodimerization activity
- GO:0051301 cell division
- GO:0051974 negative regulation of telomerase activity
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of TRF1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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