TOP1 antibody | CPTC8

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Anti TOP1 Antibody, clone CPTC8 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti TOP1 Antibody, clone CPTC8 (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti TOP1 Antibody, clone CPTC8 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with TOP1 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 118 second exposure. Arrow points to TOP1 (molecular weight 100 kDa)

Anti TOP1 Antibody, clone CPTC8 (PrecisionAb Monoclonal Antibody) gallery image 2 — Immunoprecipitation of TOP1 from K562 whole cell lysate. The Input lane is 10% input; the TOP1-IP lane is the immunoprecipitation with the TOP1 antibody (2.5 µg, **VMA00559**); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG (2.5 µg, MCA691). Western blot analysis was performed using TOP1 antibody (1/1,000, **VMA00559**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, 1705047) and visualized on the ChemiDoc MP. Arrow points to TOP1.

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Mouse anti TOP1

Product Type: PrecisionAb Monoclonal
Clone: CPTC8
Isotype: IgG2b
Format: Purified
Specificity: TOP1

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Mouse anti Human TOP1 antibody recognizes topoisomerase (DNA) I.

The TOP1 gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22 (provided by RefSeq, Jul 2008).

Mouse anti Human TOP1 antibody detects a band of 100 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti TOP1 detects a band of approximately 100 kDa in K562 cell lysates.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography on Protein G from ascites.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: Recombinant protein of human TOP1
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml.
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunoprecipitation
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.