TNF Alpha antibody | D2E7

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Human anti TNF Alpha (Adalimumab Biosimilar)

Anti-TNF Alpha Antibody is a non-therapeutic biosimilar of the monoclonal antibody drug adalimumab (Humira) for research use. It can be used in bioanalytical PK and ADA assays and for studying biological pathways affected by the drug.

Product Type
Monoclonal Antibody
Clone
D2E7
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA6088
Datasheet
E
MSDS
0.2 mg loader

Human Anti-TNF alpha antibody, clone D2E7 is a research grade biosimilar of the monoclonal antibody drug adalimumab. It is a recombinant human IgG1 kappa antibody with variable regions from the therapeutic adalimumab antibody clone D2E7.

Human Anti-TNF alpha antibody, clone D2E7 is for research use only. It is suitable as a reference standard in a pharmacokinetic (PK) bridging ELISA with anti-adalimumab antibodies, for example HCA202 and HCA203. It can also be used as capture and detection reagent in an anti-drug antibody (ADA) bridging ELISA, with an anti-adalimumab antibody in IgG1 format as reference standard, for example HCA203, HCA204 or HCA205, or an anti-adalimumab antibody in IgE format as reference standard, for example HCA271.

The therapeutic antibody adalimumab (Humira) is a fully human monoclonal antibody (IgG1 kappa) approved for the treatment of ankylosing spondylitis, Crohn’s disease, psoriasis and several forms of arthritis. The antibody is specific for TNF alpha, and acts by blocking the activation of TNF receptors by TNF alpha. This inactivation down-regulates the inflammatory reactions associated with autoimmune diseases.

This product is NOT FOR THERAPEUTIC USE.

View a summary of supporting anti-adalimumab antibody products

Product Details

Target Species
Human
Product Form
Human IgG1 (kappa) antibody - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Proclin 300
Immunogen
Human TNFα
Purity
>98% by SDS PAGE
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use
Guarantee
12 months from date of despatch

More Information

Licensed Use
For in vitro research purposes only. Research grade biosimilar. Not for use in therapeutic or diagnostic procedures for humans or animals.
Regulatory
For research purposes only

Applications of TNF Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Hispec Assay Diluent BUF049A E IY 50 ml
Human anti Adalimumab HCA202 E 0.1 mg loader
Human anti Adalimumab HCA203 E 0.1 mg loader
Human anti Adalimumab HCA204 E 0.1 mg loader
Human anti Adalimumab:HRP HCA204P E 0.1 mg loader
Human anti Adalimumab HCA205 E 0.1 mg loader
Human anti Adalimumab (Drug/Target Complex) HCA206 E 0.1 mg loader
Human anti Adalimumab (Drug/Target Complex) HCA207 E 0.1 mg loader
Human anti Adalimumab (Drug/Target Complex) HCA231 E 0.1 mg loader
Human anti Adalimumab (Drug/Target Complex) HCA232 E 0.1 mg loader
Human anti Adalimumab (Drug/Target Complex):HRP HCA232P E 0.1 mg loader
Human anti Adalimumab HCA271 E 0.1 mg loader
LYNX Rapid HRP Antibody Conjugation Kit LNK001P CJ 1 Conjugation For 400µg Antibody
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