TNF Alpha antibody | B-D9

100% Secure

Anti Human TNF Alpha Antibody, clone B-D9 gallery image 1 — Figure A. Unstimulated cells were stained with Alexa Fluor 700 conjugated Mouse anti Human CD3 (MCA463A700) and FITC conjugated anti Human TNF alpha (**MCA1395F**). Figure B. Cells stimulated with Cell Stimulation Reagent containing Brefeldin A (BUF077A) for 6 hours were stained with Alexa Fluor 700 conjugated Mouse anti Human CD3 (MCA463A700) and FITC conjugated Mouse anti Human TNF alpha (MCA1395F). All experiments performed on red cell lysed human blood gated on lymphoid cells in the presence of 10% human serum. Data acquired on the ZE5™ Cell Analyzer

Mouse anti Human TNF Alpha:FITC

Product Type: Monoclonal Antibody
Clone: B-D9
Isotype: IgG1

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
MCA1395F Datasheet	F* SDS	100 Tests/1ml	Log in		Get Quote

Mouse anti Human TNF alpha antibody, clone B-D9 recognizes human TNF-alpha, both natural and recombinant forms. The FITC conjugated reagent is designed for the detection and enumeration of TNF-alpha expressing cells in flow cytometric assays following cell membrane permeabilisation.

Product Details

Target Species: Human
Product Form: Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC)
Preparation: Purified IgG prepared by ion exchange chromatography
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.1% Sodium Azide, 5% Bovine Serum Albumin.
Immunogen: Recombinant human TNF-alpha.
Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63 Ag8.653 myeloma cell line.

Storage Information

Storage: This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Guarantee: 12 months from date of despatch

More Information

UniProt: P01375
Entrez Gene: TNF
GO Terms: GO:0000060 protein import into nucleus, translocation; GO:0000185 activation of MAPKKK activity; GO:0000187 activation of MAPK activity; GO:0001891 phagocytic cup; GO:0002439 chronic inflammatory response to antigenic stimulus; GO:0002740 negative regulation of cytokine secretion involved in immune response; GO:0009615 response to virus; GO:0005887 integral to plasma membrane; GO:0005125 cytokine activity; GO:0005164 tumor necrosis factor receptor binding; GO:0005615 extracellular space; GO:0006916 anti-apoptosis; GO:0006919 activation of caspase activity; GO:0006927 transformed cell apoptosis; GO:0050796 regulation of insulin secretion; GO:0042802 identical protein binding; GO:0009651 response to salt stress; GO:0009897 external side of plasma membrane; GO:0010843 promoter binding; GO:0010888 negative regulation of lipid storage; GO:0016563 transcription activator activity; GO:0016564 transcription repressor activity; GO:0030730 sequestering of triglyceride; GO:0031334 positive regulation of protein complex assembly; GO:0031622 positive regulation of fever generation; GO:0032582 negative regulation of gene-specific transcription; GO:0032715 negative regulation of interleukin-6 production; GO:0032722 positive regulation of chemokine production; GO:0032800 receptor biosynthetic process; GO:0033138 positive regulation of peptidyl-serine phosphorylation; GO:0033209 tumor necrosis factor-mediated signaling pathway; GO:0034116 positive regulation of heterotypic cell-cell adhesion; GO:0042346 positive regulation of NF-kappaB import into nucleus; GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade; GO:0043193 positive regulation of gene-specific transcription; GO:0043243 positive regulation of protein complex disassembly; GO:0045071 negative regulation of viral genome replication; GO:0045080 positive regulation of chemokine biosynthetic process; GO:0045121 membrane raft; GO:0045429 positive regulation of nitric oxide biosynthetic process; GO:0045599 negative regulation of fat cell differentiation; GO:0045672 positive regulation of osteoclast differentiation; GO:0048661 positive regulation of smooth muscle cell proliferation; GO:0050715 positive regulation of cytokine secretion; GO:0050901 leukocyte tethering or rolling; GO:0050995 negative regulation of lipid catabolic process; GO:0051044 positive regulation of membrane protein ectodomain proteolysis; GO:0051092 positive regulation of NF-kappaB transcription factor activity; GO:0051384 response to glucocorticoid stimulus; GO:0055037 recycling endosome; GO:0060555 induction of necroptosis by extracellular signals; GO:0060557 positive regulation of vitamin D biosynthetic process; GO:0060559 positive regulation of calcidiol 1-monooxygenase activity; GO:0061048 negative regulation of branching involved in lung morphogenesis; GO:0070265 necrotic cell death; GO:0070374 positive regulation of ERK1 and ERK2 cascade
Regulatory: For research purposes only

Applications of TNF Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry ¹		Neat	1/10

¹ Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm^™ (BUF09A) for this purpose.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 5 x 10⁵ cells in 50μl

Negative Isotype Controls Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Mouse IgG1 Negative Control:FITC	MCA928F	F	100 Tests	Log in		Get Quote

Useful Reagents Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Human Seroblock	BUF070A	F	50 Test	Log in		Get Quote
Human Seroblock	BUF070B	F	200 Test	Log in		Get Quote

Product Specific References

References for TNF Alpha antibody

North, M.E. et al. (1998) Primary defect in CD8+ lymphocytes in the antibody deficiency disease (common variable immunodeficiency): abnormalities in intracellular production of interferon-gamma (IFN-gamma) in CD28+ ('cytotoxic') and CD28- ('suppressor') CD8+ subsets.
Clin Exp Immunol. 111 (1): 70-5.
North, M.E. et al. (1996) Intracellular cytokine production by human CD4+ and CD8+ T cells from normal and immunodeficient donors using directly conjugated anti-cytokine antibodies and three-colour flow cytometry.
Clin Exp Immunol. 105: 517-22.
Sewell, W.A. et al. (1997) Determination of intracellular cytokines by flow-cytometry following whole-blood culture.
J Immunol Methods. 209: 67-74.
Sewell, W.A. et al. (1999) In vivo modulation of cytokine synthesis by intravenous immunoglobulin.
Clin Exp Immunol. 116: 509-15.
Hart, A.L. et al. (2004) Prospective evaluation of intestinal homing memory T cells in ulcerative colitis
Inflamm Bowel Dis. 10: 496-503.

Fluorescent Spectraviewer

Watch the Tool Tutorial Video ▸

How to Use the Spectraviewer