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TNF Alpha antibody | B-D9

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Anti Human TNF Alpha Antibody, clone B-D9 gallery image 1
Figure A. Unstimulated cells were stained with Alexa Fluor 700 conjugated Mouse anti Human CD3 (MCA463A700) and FITC conjugated anti Human TNF alpha (MCA1395F). Figure B. Cells stimulated with Cell Stimulation Reagent containing Brefeldin A (BUF077A) for 6 hours were stained with Alexa Fluor 700 conjugated Mouse anti Human CD3 (MCA463A700) and FITC conjugated Mouse anti Human TNF alpha (MCA1395F). All experiments performed on red cell lysed human blood gated on lymphoid cells in the presence of 10% human serum. Data acquired on the ZE5™ Cell Analyzer

Mouse anti Human TNF Alpha:FITC

Product Type
Monoclonal Antibody
Clone
B-D9
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA1395F
Datasheet
F*
SDS
100 Tests/1ml loader

Mouse anti Human TNF alpha antibody, clone B-D9 recognizes human TNF-alpha, both natural and recombinant forms. The FITC conjugated reagent is designed for the detection and enumeration of TNF-alpha expressing cells in flow cytometric assays following cell membrane permeabilisation.

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC)
Preparation
Purified IgG prepared by ion exchange chromatography
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.1% Sodium Azide, 5% Bovine Serum Albumin.
Immunogen
Recombinant human TNF-alpha.
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63 Ag8.653 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P01375
Entrez Gene
TNF
GO Terms
GO:0000060 protein import into nucleus, translocation
GO:0000185 activation of MAPKKK activity
GO:0000187 activation of MAPK activity
GO:0001891 phagocytic cup
GO:0002439 chronic inflammatory response to antigenic stimulus
GO:0002740 negative regulation of cytokine secretion involved in immune response
GO:0009615 response to virus
GO:0005887 integral to plasma membrane
GO:0005125 cytokine activity
GO:0005164 tumor necrosis factor receptor binding
GO:0005615 extracellular space
GO:0006916 anti-apoptosis
GO:0006919 activation of caspase activity
GO:0006927 transformed cell apoptosis
GO:0050796 regulation of insulin secretion
GO:0042802 identical protein binding
GO:0009651 response to salt stress
GO:0009897 external side of plasma membrane
GO:0010843 promoter binding
GO:0010888 negative regulation of lipid storage
GO:0016563 transcription activator activity
GO:0016564 transcription repressor activity
GO:0030730 sequestering of triglyceride
GO:0031334 positive regulation of protein complex assembly
GO:0031622 positive regulation of fever generation
GO:0032582 negative regulation of gene-specific transcription
GO:0032715 negative regulation of interleukin-6 production
GO:0032722 positive regulation of chemokine production
GO:0032800 receptor biosynthetic process
GO:0033138 positive regulation of peptidyl-serine phosphorylation
GO:0033209 tumor necrosis factor-mediated signaling pathway
GO:0034116 positive regulation of heterotypic cell-cell adhesion
GO:0042346 positive regulation of NF-kappaB import into nucleus
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0043193 positive regulation of gene-specific transcription
GO:0043243 positive regulation of protein complex disassembly
GO:0045071 negative regulation of viral genome replication
GO:0045080 positive regulation of chemokine biosynthetic process
GO:0045121 membrane raft
GO:0045429 positive regulation of nitric oxide biosynthetic process
GO:0045599 negative regulation of fat cell differentiation
GO:0045672 positive regulation of osteoclast differentiation
GO:0048661 positive regulation of smooth muscle cell proliferation
GO:0050715 positive regulation of cytokine secretion
GO:0050901 leukocyte tethering or rolling
GO:0050995 negative regulation of lipid catabolic process
GO:0051044 positive regulation of membrane protein ectodomain proteolysis
GO:0051092 positive regulation of NF-kappaB transcription factor activity
GO:0051384 response to glucocorticoid stimulus
GO:0055037 recycling endosome
GO:0060555 induction of necroptosis by extracellular signals
GO:0060557 positive regulation of vitamin D biosynthetic process
GO:0060559 positive regulation of calcidiol 1-monooxygenase activity
GO:0061048 negative regulation of branching involved in lung morphogenesis
GO:0070265 necrotic cell death
GO:0070374 positive regulation of ERK1 and ERK2 cascade
Regulatory
For research purposes only

Applications of TNF Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 Neat 1/10
  1. 1 Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (BUF09A) for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 5 x 105 cells in 50μl
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test

Product Specific References

References for TNF Alpha antibody

  1. North, M.E. et al. (1998) Primary defect in CD8+ lymphocytes in the antibody deficiency disease (common variable immunodeficiency): abnormalities in intracellular production of interferon-gamma (IFN-gamma) in CD28+ ('cytotoxic') and CD28- ('suppressor') CD8+ subsets.
    Clin Exp Immunol. 111 (1): 70-5.
  2. North, M.E. et al. (1996) Intracellular cytokine production by human CD4+ and CD8+ T cells from normal and immunodeficient donors using directly conjugated anti-cytokine antibodies and three-colour flow cytometry.
    Clin Exp Immunol. 105: 517-22.
  3. Sewell, W.A. et al. (1997) Determination of intracellular cytokines by flow-cytometry following whole-blood culture.
    J Immunol Methods. 209: 67-74.
  4. Sewell, W.A. et al. (1999) In vivo modulation of cytokine synthesis by intravenous immunoglobulin.
    Clin Exp Immunol. 116: 509-15.
  5. Hart, A.L. et al. (2004) Prospective evaluation of intestinal homing memory T cells in ulcerative colitis
    Inflamm Bowel Dis. 10: 496-503.
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