TNF Alpha antibody | B-D9
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|Mouse anti Human TNF alpha antibody, clone B-D9 recognizes human TNF-alpha, both natural and recombinant forms. The FITC conjugated reagent is designed for the detection and enumeration of TNF-alpha expressing cells in flow cytometric assays following cell membrane permeabilisation.|
- Target Species
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC)
- Purified IgG prepared by ion exchange chromatography
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.1% Sodium Azide, 5% Bovine Serum Albumin.
- Recombinant human TNF-alpha.
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63 Ag8.653 myeloma cell line.
- Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
|Application Name||Verified||Min Dilution||Max Dilution|
|Flow Cytometry 1||Neat||1/10|
- 1 Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (BUF09A) for this purpose.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 5 x 105 cells in 50μl
References for TNF Alpha antibody
North, M.E. et al. (1998) Primary defect in CD8+ lymphocytes in the antibody deficiency disease (common variable immunodeficiency): abnormalities in intracellular production of interferon-gamma (IFN-gamma) in CD28+ ('cytotoxic') and CD28- ('suppressor') CD8+ subsets.
Clin Exp Immunol. 111 (1): 70-5.
North, M.E. et al. (1996) Intracellular cytokine production by human CD4+ and CD8+ T cells from normal and immunodeficient donors using directly conjugated anti-cytokine antibodies and three-colour flow cytometry.
Clin Exp Immunol. 105: 517-22.
Sewell, W.A. et al. (1997) Determination of intracellular cytokines by flow-cytometry following whole-blood culture.
J Immunol Methods. 209: 67-74.
Sewell, W.A. et al. (1999) In vivo modulation of cytokine synthesis by intravenous immunoglobulin.
Clin Exp Immunol. 116: 509-15.
Hart, A.L. et al. (2004) Prospective evaluation of intestinal homing memory T cells in ulcerative colitis
Inflamm Bowel Dis. 10: 496-503.
- Entrez Gene
- GO Terms
- GO:0000060 protein import into nucleus, translocation
- GO:0000185 activation of MAPKKK activity
- GO:0000187 activation of MAPK activity
- GO:0001891 phagocytic cup
- GO:0002439 chronic inflammatory response to antigenic stimulus
- GO:0002740 negative regulation of cytokine secretion involved in immune response
- GO:0009615 response to virus
- GO:0005887 integral to plasma membrane
- GO:0005125 cytokine activity
- View More GO Terms
- GO:0005164 tumor necrosis factor receptor binding
- GO:0005615 extracellular space
- GO:0006916 anti-apoptosis
- GO:0006919 activation of caspase activity
- GO:0006927 transformed cell apoptosis
- GO:0050796 regulation of insulin secretion
- GO:0042802 identical protein binding
- GO:0009651 response to salt stress
- GO:0009897 external side of plasma membrane
- GO:0010843 promoter binding
- GO:0010888 negative regulation of lipid storage
- GO:0016563 transcription activator activity
- GO:0016564 transcription repressor activity
- GO:0030730 sequestering of triglyceride
- GO:0031334 positive regulation of protein complex assembly
- GO:0031622 positive regulation of fever generation
- GO:0032582 negative regulation of gene-specific transcription
- GO:0032715 negative regulation of interleukin-6 production
- GO:0032722 positive regulation of chemokine production
- GO:0032800 receptor biosynthetic process
- GO:0033138 positive regulation of peptidyl-serine phosphorylation
- GO:0033209 tumor necrosis factor-mediated signaling pathway
- GO:0034116 positive regulation of heterotypic cell-cell adhesion
- GO:0042346 positive regulation of NF-kappaB import into nucleus
- GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
- GO:0043193 positive regulation of gene-specific transcription
- GO:0043243 positive regulation of protein complex disassembly
- GO:0045071 negative regulation of viral genome replication
- GO:0045080 positive regulation of chemokine biosynthetic process
- GO:0045121 membrane raft
- GO:0045429 positive regulation of nitric oxide biosynthetic process
- GO:0045599 negative regulation of fat cell differentiation
- GO:0045672 positive regulation of osteoclast differentiation
- GO:0048661 positive regulation of smooth muscle cell proliferation
- GO:0050715 positive regulation of cytokine secretion
- GO:0050901 leukocyte tethering or rolling
- GO:0050995 negative regulation of lipid catabolic process
- GO:0051044 positive regulation of membrane protein ectodomain proteolysis
- GO:0051092 positive regulation of NF-kappaB transcription factor activity
- GO:0051384 response to glucocorticoid stimulus
- GO:0055037 recycling endosome
- GO:0060555 induction of necroptosis by extracellular signals
- GO:0060557 positive regulation of vitamin D biosynthetic process
- GO:0060559 positive regulation of calcidiol 1-monooxygenase activity
- GO:0061048 negative regulation of branching involved in lung morphogenesis
- GO:0070265 necrotic cell death
- GO:0070374 positive regulation of ERK1 and ERK2 cascade
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