TNF Alpha antibody | 4C6-H6

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Mouse anti Human TNF Alpha

Product Type: Monoclonal Antibody
Clone: 4C6-H6
Isotype: IgM

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
MCA1385 Datasheet	E F* IP P SDS	2 ml	Log in		Get Quote

Mouse anti Human TNF alpha antibody clone 4C6-H6 recognizes human Tumor necrosis factor, also known as TNF alpha, Cachectin or Tumor necrosis factor ligand superfamily member 2. TNFα is a 233 amino acid ~22kDa type II transmembrane protein with a single potential glycosylation site (Uniprot P01375: Takakura-Yamamoto et al. 1996).

Mouse anti Human TNF alpha antibody clone 4C6-H6 has been used successfully for the demonstration of TNFα expression in formalin fixed, paraffin embedded bone marrow biopsies from patients with Myelodysplastic syndrome (Molnár et al. 2000) ant has been used for evaluating the increase in TNFα expression seen in peripheral blood mononuclear cells from patients suffering from the autoinnmuse condition, Graves disease (Quadbeck et al. 2006).

Product Details

Target Species: Human
Product Form: Tissue Culture Supernatant - liquid
Preparation: Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum
Preservative Stabilisers: <0.1% Sodium Azide (NaN₃)
Immunogen: 115-130 AA sequence linked to thyroglobulin
Fusion Partners: Spleen cells from immunized BALB/c mice were fused with cells of the SP2/0-Ag14 myeloma cell line

Storage Information

Storage: This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee: 12 months from date of despatch

More Information

UniProt: P01375
Entrez Gene: TNF
GO Terms: GO:0000060 protein import into nucleus, translocation; GO:0000185 activation of MAPKKK activity; GO:0000187 activation of MAPK activity; GO:0001891 phagocytic cup; GO:0002439 chronic inflammatory response to antigenic stimulus; GO:0002740 negative regulation of cytokine secretion involved in immune response; GO:0009615 response to virus; GO:0005887 integral to plasma membrane; GO:0005125 cytokine activity; GO:0005164 tumor necrosis factor receptor binding; GO:0005615 extracellular space; GO:0006916 anti-apoptosis; GO:0006919 activation of caspase activity; GO:0006927 transformed cell apoptosis; GO:0050796 regulation of insulin secretion; GO:0042802 identical protein binding; GO:0009651 response to salt stress; GO:0009897 external side of plasma membrane; GO:0010843 promoter binding; GO:0010888 negative regulation of lipid storage; GO:0016563 transcription activator activity; GO:0016564 transcription repressor activity; GO:0030730 sequestering of triglyceride; GO:0031334 positive regulation of protein complex assembly; GO:0031622 positive regulation of fever generation; GO:0032582 negative regulation of gene-specific transcription; GO:0032715 negative regulation of interleukin-6 production; GO:0032722 positive regulation of chemokine production; GO:0032800 receptor biosynthetic process; GO:0033138 positive regulation of peptidyl-serine phosphorylation; GO:0033209 tumor necrosis factor-mediated signaling pathway; GO:0034116 positive regulation of heterotypic cell-cell adhesion; GO:0042346 positive regulation of NF-kappaB import into nucleus; GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade; GO:0043193 positive regulation of gene-specific transcription; GO:0043243 positive regulation of protein complex disassembly; GO:0045071 negative regulation of viral genome replication; GO:0045080 positive regulation of chemokine biosynthetic process; GO:0045121 membrane raft; GO:0045429 positive regulation of nitric oxide biosynthetic process; GO:0045599 negative regulation of fat cell differentiation; GO:0045672 positive regulation of osteoclast differentiation; GO:0048661 positive regulation of smooth muscle cell proliferation; GO:0050715 positive regulation of cytokine secretion; GO:0050901 leukocyte tethering or rolling; GO:0050995 negative regulation of lipid catabolic process; GO:0051044 positive regulation of membrane protein ectodomain proteolysis; GO:0051092 positive regulation of NF-kappaB transcription factor activity; GO:0051384 response to glucocorticoid stimulus; GO:0055037 recycling endosome; GO:0060555 induction of necroptosis by extracellular signals; GO:0060557 positive regulation of vitamin D biosynthetic process; GO:0060559 positive regulation of calcidiol 1-monooxygenase activity; GO:0061048 negative regulation of branching involved in lung morphogenesis; GO:0070265 necrotic cell death; GO:0070374 positive regulation of ERK1 and ERK2 cascade
Regulatory: For research purposes only

Applications of TNF Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA	1/2	1/10
Flow Cytometry ¹		Neat
Immunohistology - Paraffin
Immunoprecipitation

¹Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm (BUF09) for this purpose.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	Quantity
Human anti Mouse IgM:FITC	HCA040F	F	0.1 mg	Log in	Get Quote
Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed)	STAR138A	C E P WB	1 ml	Log in	Get Quote
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	C E WB	1 mg	Log in	Get Quote
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	E	1 mg	Log in	Get Quote

Negative Isotype Controls Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Mouse IgM Negative Control	MCA692	F	100 Tests	Log in		Get Quote

Product Specific References

References for TNF Alpha antibody

Bebök Z et al. (1994) Prognostic relevance of transforming growth factor alpha (TGF-alpha) and tumor necrosis factor alpha (TNF-alpha) detected in breast cancer tissues by immunohistochemistry.
Breast Cancer Res Treat. 29 (3): 229-35.
Molnár L et al. (2000) Detection of TNFalpha expression in the bone marrow and determination of TNFalpha production of peripheral blood mononuclear cells in myelodysplastic syndrome.
Pathol Oncol Res. 6 (1): 18-23.
Togo, T. et al. (2001) Glial involvement in the degeneration process of Lewy body-bearing neurons and the degradation process of Lewy bodies in brains of dementia with Lewy bodies.
J Neurol Sci. 184: 71-5.
Brown, H. et al. (1999) Cytokine expression in the brain in human cerebral malaria.
J Infect Dis. 180: 1742-6.
Quadbeck, B. et al. (2006) Dysregulation of TNF/TNFR superfamily members: a systemic link between intra- and extrathyroidal manifestations in Graves' disease.
Scand J Immunol. 64: 523-30.

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