Mouse anti Human TNF alpha antibody clone 4C6-H6
recognizes human Tumor necrosis factor, also known as TNF alpha, Cachectin or Tumor necrosis factor ligand superfamily member 2. TNFα is a 233 amino acid ~22kDa type II transmembrane protein with a single potential glycosylation site (Uniprot P01375
: Takakura-Yamamoto et al. 1996
Mouse anti Human TNF alpha antibody clone 4C6-H6 has been used successfully for the demonstration of TNFα expression in formalin fixed, paraffin embedded bone marrow biopsies from patients with Myelodysplastic syndrome (Molnár et al. 2000
) ant has been used for evaluating the increase in TNFα expression seen in peripheral blood mononuclear cells from patients suffering from the autoinnmuse condition, Graves disease (Quadbeck et al. 2006
- Target Species
- Product Form
- Tissue Culture Supernatant - liquid
- Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum
- Preservative Stabilisers
- 115-130 AA sequence linked to thyroglobulin
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the SP2/0-Ag14 myeloma cell line
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
protein import into nucleus, translocation
activation of MAPKKK activity
activation of MAPK activity
chronic inflammatory response to antigenic stimulus
negative regulation of cytokine secretion involved in immune response
response to virus
integral to plasma membrane
tumor necrosis factor receptor binding
activation of caspase activity
transformed cell apoptosis
regulation of insulin secretion
identical protein binding
response to salt stress
external side of plasma membrane
negative regulation of lipid storage
transcription activator activity
transcription repressor activity
sequestering of triglyceride
positive regulation of protein complex assembly
positive regulation of fever generation
negative regulation of gene-specific transcription
negative regulation of interleukin-6 production
positive regulation of chemokine production
receptor biosynthetic process
positive regulation of peptidyl-serine phosphorylation
tumor necrosis factor-mediated signaling pathway
positive regulation of heterotypic cell-cell adhesion
positive regulation of NF-kappaB import into nucleus
positive regulation of I-kappaB kinase/NF-kappaB cascade
positive regulation of gene-specific transcription
positive regulation of protein complex disassembly
negative regulation of viral genome replication
positive regulation of chemokine biosynthetic process
positive regulation of nitric oxide biosynthetic process
negative regulation of fat cell differentiation
positive regulation of osteoclast differentiation
positive regulation of smooth muscle cell proliferation
positive regulation of cytokine secretion
leukocyte tethering or rolling
negative regulation of lipid catabolic process
positive regulation of membrane protein ectodomain proteolysis
positive regulation of NF-kappaB transcription factor activity
response to glucocorticoid stimulus
induction of necroptosis by extracellular signals
positive regulation of vitamin D biosynthetic process
positive regulation of calcidiol 1-monooxygenase activity
negative regulation of branching involved in lung morphogenesis
necrotic cell death
positive regulation of ERK1 and ERK2 cascade
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of TNF Alpha antibody
|Flow Cytometry 1
|Immunohistology - Paraffin
- 1Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm (BUF09) for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
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Secondary Antibodies Available
Negative Isotype Controls Available
Product Specific References
References for TNF Alpha antibody
Bebök Z et al. (1994) Prognostic relevance of transforming growth factor alpha (TGF-alpha) and tumor necrosis factor alpha (TNF-alpha) detected in breast cancer tissues by immunohistochemistry.
Breast Cancer Res Treat. 29 (3): 229-35.
Molnár L et al. (2000) Detection of TNFalpha expression in the bone marrow and determination of TNFalpha production of peripheral blood mononuclear cells in myelodysplastic syndrome.
Pathol Oncol Res. 6 (1): 18-23.
Togo, T. et al. (2001) Glial involvement in the degeneration process of Lewy body-bearing neurons and the degradation process of Lewy bodies in brains of dementia with Lewy bodies.
J Neurol Sci. 184: 71-5.
Brown, H. et al. (1999) Cytokine expression in the brain in human cerebral malaria.
J Infect Dis. 180: 1742-6.
Quadbeck, B. et al. (2006) Dysregulation of TNF/TNFR superfamily members: a systemic link between intra- and extrathyroidal manifestations in Graves' disease.
Scand J Immunol. 64: 523-30.