TMS1 antibody | OTI1A2

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Mouse anti TMS1

TMS1 antibody recognizes apoptosis-associated speck-like protein containing a CARD, also known as caspase recruitment domain-containing protein 5 or target of methylation-induced silencing 1. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Clone
OTI1A2
Isotype
IgG1
Format
Purified
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00183 WB 100 µl
Mouse anti Human TMS1 antibody recognizes target of methylation-induced silencing 1 (TMS1), also known as apoptosis-associated speck-like protein containing a CARD and caspase recruitment domain-containing protein 5.

The TMS1 (PYCARD) gene encodes TMS1, an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for PYCARD (provided by RefSeq, Jul 2008).

Mouse anti Human TMS1 antibody detects a band of 22 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti TMS1 detects a band of approximately 22 kDa in THP-1 cell lysates.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant human TMS1 protein produced in E. coli
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
Q9ULZ3 Related reagents
Entrez Gene
PYCARD Related reagents
GO Terms
GO:0004197 cysteine-type endopeptidase activity
GO:0006508 proteolysis
GO:0006917 induction of apoptosis
GO:0006919 activation of caspase activity
GO:0008385 IkappaB kinase complex
GO:0008656 caspase activator activity
GO:0032090 Pyrin domain binding
GO:0033209 tumor necrosis factor-mediated signaling pathway
GO:0042803 protein homodimerization activity
GO:0050718 positive regulation of interleukin-1 beta secretion
GO:0051092 positive regulation of NF-kappaB transcription factor activity
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of TMS1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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