TKT antibody | OTI5H3

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Mouse anti TKT

TKT antibody recognizes the enzyme transketolase, also known as epididymis luminal protein 107. This antibody has been extensively validated for western blotting using whole cell lysates.

TKT Antibody with Control Lysate

TKT antibody recognizes the enzyme transketolase, also known as epididymis luminal protein 107. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Clone
OTI5H3
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00170 WB 100 µl
VMA00170KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human TKT antibody recognizes TKT, also known as epididymis luminal protein 107.

The TKT gene encodes a thiamine-dependent enzyme which plays a role in the channeling of excess sugar phosphates to glycolysis in the pentose phosphate pathway. Alternatively spliced transcript variants encoding multiple isoforms have been observed for TKT (provided by RefSeq, Apr 2012).

Mouse anti Human TKT antibody detects a band of 68 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites.
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant human TKT (NP_001055) produced in HEK293T cells

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P29401 Related reagents
Entrez Gene
TKT Related reagents
GO Terms
GO:0005515 protein binding
GO:0005829 cytosol
GO:0004802 transketolase activity
GO:0046872 metal ion binding
GO:0005999 xylulose biosynthetic process
GO:0006098 pentose-phosphate shunt
GO:0006112 energy reserve metabolic process
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of TKT antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti TKT detects a band of approximately 68 kDa in HeLa cell lysates.
Western Blotting
Anti TKT detects a band of approximately 68 kDa in HeLa cell lysates.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB