Tissue Factor Pathway Inhibitor antibody | OTI4H9
Mouse anti Tissue Factor Pathway Inhibitor
Tissue factor pathway inhibitor antibody recognizes tissue factor pathway inhibitor, also known as TFPI, anti-convertin or extrinsic pathway inhibitor. This antibody has been extensively validated for western blotting using whole cell lysates.
Tissue Factor Pathway Inhibitor Antibody with Control Lysate
Tissue factor pathway inhibitor antibody recognizes tissue factor pathway inhibitor, also known as TFPI, anti-convertin or extrinsic pathway inhibitor. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Monoclonal
- Clone
- OTI4H9
- Isotype
- IgG2a
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00293 | WB | 100 µl | ||||
| VMA00293KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human tissue factor pathway inhibitor antibody recognizes tissue factor pathway inhibitor, also known as anti-convertin and extrinsic pathway inhibitor.
Encoded by the TFPI gene, tissue factor pathway inhibitor is a protease inhibitor that regulates the tissue factor (TF)-dependent pathway of blood coagulation. The coagulation process initiates with the formation of a factor VIIa-TF complex, which proteolytically activates additional proteases (factors IX and X) and ultimately leads to the formation of a fibrin clot. The product of TFPI inhibits the activated factor X and VIIa-TF proteases in an autoregulatory loop. The encoded protein is glycosylated and predominantly found in the vascular endothelium and plasma in both free forms and complexed with plasma lipoproteins. Several alternatively spliced transcript variants of TFPI have been described, but the full-length nature of some of these variants has not been confirmed (provided by RefSeq, Jul 2008).
Mouse anti Human tissue factor pathway inhibitor antibody detects a band of 40-45 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Encoded by the TFPI gene, tissue factor pathway inhibitor is a protease inhibitor that regulates the tissue factor (TF)-dependent pathway of blood coagulation. The coagulation process initiates with the formation of a factor VIIa-TF complex, which proteolytically activates additional proteases (factors IX and X) and ultimately leads to the formation of a fibrin clot. The product of TFPI inhibits the activated factor X and VIIa-TF proteases in an autoregulatory loop. The encoded protein is glycosylated and predominantly found in the vascular endothelium and plasma in both free forms and complexed with plasma lipoproteins. Several alternatively spliced transcript variants of TFPI have been described, but the full-length nature of some of these variants has not been confirmed (provided by RefSeq, Jul 2008).
Mouse anti Human tissue factor pathway inhibitor antibody detects a band of 40-45 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti tissue factor pathway inhibitor detects a band of approximately 40-45 kDa in HepG2 cell lysates
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography from tissue culture supernatant
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatography from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- Recombinant protein corresponding to amino acids 29-304 of human tissue factor pathway inhibitor (NP_006278) produced in E. coli
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HepG2 lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
More Information
- UniProt
- P10646 Related reagents
- Entrez Gene
- TFPI Related reagents
- GO Terms
- GO:0005886 plasma membrane
- GO:0004867 serine-type endopeptidase inhibitor activity
- GO:0005615 extracellular space
- GO:0007598 blood coagulation, extrinsic pathway
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of Tissue Factor Pathway Inhibitor antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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