Tissue Factor Pathway Inhibitor antibody | OTI4H9

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Mouse anti Tissue Factor Pathway Inhibitor

Tissue factor pathway inhibitor antibody recognizes tissue factor pathway inhibitor, also known as TFPI, anti-convertin or extrinsic pathway inhibitor. This antibody has been extensively validated for western blotting using whole cell lysates.

Tissue Factor Pathway Inhibitor Antibody with Control Lysate

Tissue factor pathway inhibitor antibody recognizes tissue factor pathway inhibitor, also known as TFPI, anti-convertin or extrinsic pathway inhibitor. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Clone
OTI4H9
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00293 WB 100 µl
VMA00293KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human tissue factor pathway inhibitor antibody recognizes tissue factor pathway inhibitor, also known as anti-convertin and extrinsic pathway inhibitor.

Encoded by the TFPI gene, tissue factor pathway inhibitor is a protease inhibitor that regulates the tissue factor (TF)-dependent pathway of blood coagulation. The coagulation process initiates with the formation of a factor VIIa-TF complex, which proteolytically activates additional proteases (factors IX and X) and ultimately leads to the formation of a fibrin clot. The product of TFPI inhibits the activated factor X and VIIa-TF proteases in an autoregulatory loop. The encoded protein is glycosylated and predominantly found in the vascular endothelium and plasma in both free forms and complexed with plasma lipoproteins. Several alternatively spliced transcript variants of TFPI have been described, but the full-length nature of some of these variants has not been confirmed (provided by RefSeq, Jul 2008).

Mouse anti Human tissue factor pathway inhibitor antibody detects a band of 40-45 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from tissue culture supernatant
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Recombinant protein corresponding to amino acids 29-304 of human tissue factor pathway inhibitor (NP_006278) produced in E. coli

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P10646 Related reagents
Entrez Gene
TFPI Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0004867 serine-type endopeptidase inhibitor activity
GO:0005615 extracellular space
GO:0007598 blood coagulation, extrinsic pathway
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Tissue Factor Pathway Inhibitor antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti tissue factor pathway inhibitor detects a band of approximately 40-45 kDa in HepG2 cell lysates
Western Blotting
Anti tissue factor pathway inhibitor detects a band of approximately 40-45 kDa in HepG2 cell lysates
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB