TIMP-2 antibody | D08-1D4
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Mouse anti Human TIMP-2, clone D08-1D4, recognizes the human tissue matrix metalloprotease inhibitor (TIMP)-2. TIMP-2 is a member of the TIMP family, which has 4 members (TIMP-1, TIMP-2, TIMP-3, TIMP-4) and consists of 220 amino acids with a molcular weight of 22 kDa.
TIMP-2 is a inhibitor of matrix metalloproteases but can also interact with MT1-MMP to enable the cell-surface activation of pro-MMP-2 (Lambert et al. 2004 and Baker et al. 2002). In addition to this, studies suggest that TIMP-2 selectively inhibits the growth of endothelial cells through an MMP-independent mechanism (Murphy et al. 1993 and Moses et al. 1990).
TIMP-2 is a inhibitor of matrix metalloproteases but can also interact with MT1-MMP to enable the cell-surface activation of pro-MMP-2 (Lambert et al. 2004 and Baker et al. 2002). In addition to this, studies suggest that TIMP-2 selectively inhibits the growth of endothelial cells through an MMP-independent mechanism (Murphy et al. 1993 and Moses et al. 1990).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- E. coli derived recombinant protein corresponding to aa 27-220 of human TIMP-2
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P16035
- Entrez Gene
- TIMP2
- GO Terms
- GO:0046872 metal ion binding
- GO:0008191 metalloendopeptidase inhibitor activity
- Regulatory
- For research purposes only
Applications of TIMP-2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
Product Specific References
Further Reading
-
Baker, A.H. et al. (1998) Divergent effects of tissue inhibitor of metalloproteinase-1, -2, or -3 overexpression on rat vascular smooth muscle cell invasion, proliferation, and death in vitro. TIMP-3 promotes apoptosis.
J Clin Invest. 101 (6): 1478-87. -
Moses, M.A. et al. (1990) Identification of an inhibitor of neovascularization from cartilage.
Science. 248 (4961): 1408-10. -
Murphy, A.N. et al. (1993) Tissue inhibitor of metalloproteinases-2 inhibits bFGF-induced human microvascular endothelial cell proliferation.
J Cell Physiol. 157 (2): 351-8. -
Lambert, E. et al. (2004) TIMPs as multifacial proteins.
Crit Rev Oncol Hematol. 49 (3): 187-98.
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