TIMP-1 antibody | T2

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Mouse anti Human TIMP-1

Product Type: Monoclonal Antibody
Clone: T2
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
9013-1008	E WB			0.1 mg

Mouse anti Human TIMP-1 antibody, clone T2 recognizes human Metalloproteinase inhibitor 1, also known as TIMP-1, Erythroid-potentiating activity, Fibroblast collagenase inhibitor or Tissue inhibitor of metalloproteinases 1. TIMP-1 is a 207 amino acid ~28 kDa secreted metalloproteinase inhibitor. reacting with both the reduced and non-reduced forms of TIMP-1 (UniProt: P101033).

TIMP-1 is expressed by a wide range of cells including microvascular endothelial cells, smooth muscle cells, mesangial cells and some fibroblasts. TIMP-1 inhibits matrix metalloproteinase activity by forming complexes via reversible non covalent binding. It is reported to be involved in the control of angiogenesis and tumour growth. Mouse anti Human TIMP-1 antibody, clone T2 has been shown to inhibit biological activity (Koike et al. 2002).

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Rabbit

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein A

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)

Immunogen

Human TIMP-1.

Approx. Protein Concentrations

IgG concentration 0.5 mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: P01033 Related reagents
Entrez Gene: TIMP1 Related reagents
GO Terms: GO:0005515 protein binding; GO:0002576 platelet degranulation; GO:0046872 metal ion binding; GO:0005578 proteinaceous extracellular matrix; GO:0008191 metalloendopeptidase inhibitor activity; GO:0008284 positive regulation of cell proliferation; GO:0030168 platelet activation; GO:0031093 platelet alpha granule lumen; GO:0043249 erythrocyte maturation; GO:0051045 negative regulation of membrane protein ectodomain proteolysis
Regulatory: For research purposes only

Applications of TIMP-1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA		1/100	1/400
Western Blotting		1/100	1/400

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Product Specific References

References for TIMP-1 antibody

Koike, T. et al. (2002) MT1-MMP, but not secreted MMPs, influences the migration of human microvascular endothelial cells in 3-dimensional collagen gels.
J Cell Biochem. 86: 748-58

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