Mouse anti TIGAR antibody
clone Woody-1 recognizes TIGAR also known as TP53-induced glycolysis and apoptosis regulator and TP53-induced glycolysis regulatory phosphatase. TIGAR is a fructose 2,6 bisphosphatase whose expression is induced by the transcription factor p53 in response to oxidative stress. TIGAR, as it slows down the glycolytic rate, and the accumulation of 2,6 fructose 2,6-bisphosphate has a protective role that prevents cells from "acquiring cancer hallmarks" (Bensaad et al. 2006
). In addition, TIGAR protects cells by reducing the amount of reactive oxygen species, thereby preventing DNA damage caused by reactive oxygen species (Gottlieb and Vousden, 2010
). TIGAR by mediating reactive oxygen levels has also been shown to regulate reactive oxygen induced apoptotic responses (Bensaad et al. 2006
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
- Recombinant human TIGAR (aa1-270)
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
fructose-2,6-bisphosphate 2-phosphatase activity
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Tigar antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Mouse anti TIGAR recognizes a band of ~30 kDa by western blot
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Secondary Antibodies Available
Useful Reagents Available