Thioredoxin antibody | 1850CT572.62.73

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Mouse anti Thioredoxin

Thioredoxin antibody recognizes thioredoxin, also known as ADF, ATL-derived factor, SASP, TRX, or TXN delta 3.

Thioredoxin Antibody with Control Lysate

Thioredoxin antibody recognizes thioredoxin, also known as ADF, ATL-derived factor, SASP, TRX, or TXN delta 3.

Product Type
PrecisionAb™ Monoclonal
Clone
1850CT572.62.73
Isotype
IgG1
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00708 WB 100 µl
VMA00708KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human thioredoxin antibody recognizes thioredoxin, also known as ADF, ATL-derived factor, SASP, TRX and TXN delta 3.

The protein encoded by TXN acts as a homodimer and is involved in many redox reactions. The encoded protein is active in the reversible S-nitrosylation of cysteines in certain proteins, which is part of the response to intracellular nitric oxide. This protein is found in the cytoplasm. Two transcript variants encoding different isoforms have been found for TXN (provided by RefSeq, Oct 2011).

Mouse anti Human thioredoxin antibody detects a band of 12 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Mouse anti thioredoxin detects a band of approximately 12 kDa in HepG2 cell lysates
Product Form
Purified IgG - Liquid
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Preparation
20μl Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
Recombinant protein of human thioredoxin (amino acids 1-105)
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HepG2 lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Guarantee
As supplied, 12 months from date of despatch
Guarantee
As supplied, 12 months from date of despatch

More Information

UniProt
P10599 Related reagents
Entrez Gene
TXN Related reagents
GO Terms
GO:0005515 protein binding
GO:0005576 extracellular region
GO:0005829 cytosol
GO:0007165 signal transduction
GO:0005654 nucleoplasm
GO:0022900 electron transport chain
GO:0006350 transcription
GO:0006810 transport
GO:0006928 cellular component movement
GO:0007267 cell-cell signaling
GO:0008283 cell proliferation
GO:0009314 response to radiation
GO:0015949 nucleobase, nucleoside and nucleotide interconversion
GO:0033158 regulation of protein import into nucleus, translocation
GO:0043388 positive regulation of DNA binding
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of Thioredoxin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
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