Thioredoxin 1 antibody

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Mouse anti Thioredoxin 1

Thioredoxin 1 Antibody with Control Lysate

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG2b
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00585 WB 100 µl
VMA00585KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human thioredoxin 1 antibody recognizes thioredoxin, also known as ADF, ATL-derived factor, SASP, TRX or TXN delta 3.

The protein encoded by TXN gene acts as a homodimer and is involved in many redox reactions. The encoded protein is active in the reversible S-nitrosylation of cysteines in certain proteins, which is part of the response to intracellular nitric oxide. This protein is found in the cytoplasm. Two transcript variants encoding different isoforms have been found for TXN (provided by RefSeq, Oct 2011).

Mouse anti Human thioredoxin 1 antibody detects a band of 12 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from ascites
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography Protein G from ascites
Buffer Solution
HEPES buffered saline
Buffer Solution
HEPES buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
<50% Glycerol
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
<50% Glycerol
Immunogen
E. coli-derived recombinant human thioredoxin 1

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P10599 Related reagents
Entrez Gene
TXN Related reagents
GO Terms
GO:0005515 protein binding
GO:0005576 extracellular region
GO:0005829 cytosol
GO:0007165 signal transduction
GO:0005654 nucleoplasm
GO:0022900 electron transport chain
GO:0006350 transcription
GO:0006810 transport
GO:0006928 cellular component movement
GO:0007267 cell-cell signaling
GO:0008283 cell proliferation
GO:0009314 response to radiation
GO:0015949 nucleobase, nucleoside and nucleotide interconversion
GO:0033158 regulation of protein import into nucleus, translocation
GO:0043388 positive regulation of DNA binding
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Thioredoxin 1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti thioredoxin 1 detects a band of approximately 12 kDa in HeLa cell lysates
Western Blotting
Anti thioredoxin 1 detects a band of approximately 12 kDa in HeLa cell lysates
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB