TFEB antibody | 3E1-G6

Mouse anti Human TFEB:Preservative Free

Product Type
Monoclonal Antibody
Clone
3E1-G6
Isotype
IgG1
Specificity
TFEB

Product Code Applications Pack Size List Price Your Price Qty
MCA4206Z
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
IF WB 0.1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Human TFEB antibody, clone 3E1-G6 recognizes human Transcription factor EB, also known as TFEB or Class E basic helix-loop-helix protein 35. TFEB is a 476 amino acid ~50 kDa transcription factor binding to E-Box sequences.

Two isoforms of TFEB generated by alternative splicing have been described. Mouse anti Human TFEB antibody, clone 3E1-G6 is expected to recognize the canonical isoform 1 but binding to isoform 2 which lacks part of the immunogen sequence used to generate clone 3E1-G6 has not been evaluated (UniProt : P19484).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Immunogen
Recombinant protein corresponding to aa 1-477 of human TFEB.
Approx. Protein Concentrations
Ig concentration 0.5 mg/ml
Fusion Partners
Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence 0.1 10 ug/ml
Western Blotting 0.1 10 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)

RRID
AB_2202836
UniProt
P19484
Entrez Gene
TFEB
GO Terms
GO:0001892 embryonic placenta development
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0005737 cytoplasm
GO:0006959 humoral immune response
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010843 promoter binding
GO:0030528 transcription regulator activity
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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