TFEB antibody | 3E1-G6
Mouse anti Human TFEB antibody, clone 3E1-G6 recognizes human Transcription factor EB, also known as TFEB or Class E basic helix-loop-helix protein 35. TFEB is a 476 amino acid ~50 kDa transcription factor binding to E-Box sequences.
Two isoforms of TFEB generated by alternative splicing have been described. Mouse anti Human TFEB antibody, clone 3E1-G6 is expected to recognize the canonical isoform 1 but binding to isoform 2 which lacks part of the immunogen sequence used to generate clone 3E1-G6 has not been evaluated (UniProt : P19484).
Two isoforms of TFEB generated by alternative splicing have been described. Mouse anti Human TFEB antibody, clone 3E1-G6 is expected to recognize the canonical isoform 1 but binding to isoform 2 which lacks part of the immunogen sequence used to generate clone 3E1-G6 has not been evaluated (UniProt : P19484).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Immunogen
- Recombinant protein corresponding to aa 1-477 of human TFEB.
- Approx. Protein Concentrations
- Ig concentration 0.5 mg/ml
- Fusion Partners
- Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
Storage Information
- Storage
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- P19484 Related reagents
- Entrez Gene
- TFEB Related reagents
- GO Terms
- GO:0001892 embryonic placenta development
- GO:0003700 sequence-specific DNA binding transcription factor activity
- GO:0005737 cytoplasm
- GO:0006959 humoral immune response
- GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
- GO:0010843 promoter binding
- GO:0030528 transcription regulator activity
- Regulatory
- For research purposes only
Applications of TFEB antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence | 0.1 | 10 ug/ml | |
| Western Blotting | 0.1 | 10 ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
