TdT | HT-1, HT-4, HT-8, HT-9

Mouse anti Human TdT:FITC

Product Type
Monoclonal Antibody Panel
Clone
HT-1, HT-4, HT-8, HT-9
Isotype
Cocktail
Specificity
TdT

Product Code Applications Pack Size List Price Your Price Qty
OBT0012
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F * 25 µg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Human TdT antibody panel, clones HT-1, HT-4, Ht-8 and HT-9 is a mixture of 4 monoclonal antibodies specific for human terminal deoxynucleotidyl transferase (TdT). TdT is a DNA polymerase responsible for the catalysis of non-reversible addition of deoxynucleotides to the 3' end hydroxy groups of DNA. TdT levels are enhanced in all forms of acute lymphoblastic leukaemia (ALL) and in a significant number of chronic lymphoblastic leukaemia cases (CML). TdT is not expressed in the majority of myeloid leukaemias, non-Hodgkins lymphomas or mature lymphoid leukaemias.

Target Species
Human
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Approx. Protein Concentrations
IgG concentration 50.0 ug/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
Guaranteed until date of expiry. Please see product label.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 Neat
  1. 1 Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
List Price Your Price
Description Human Seroblock
Human Seroblock BUF070B F 200 Test
List Price Your Price
Description Human Seroblock

References for TdT

  1. Wolgast, L.R. et al. (2011) Spectrin isoforms: differential expression in normal hematopoiesis and alterations in neoplastic bone marrow disorders.
    Am J Clin Pathol. 2011 Aug;136(2):300-8.

Synonyms
Terminal Deoxynucleotidyl Transferase
RRID
AB_609798
UniProt
P04053
Entrez Gene
DNTT
GO Terms
GO:0006260 DNA replication
GO:0003677 DNA binding
GO:0003887 DNA-directed DNA polymerase activity
GO:0003912 DNA nucleotidylexotransferase activity
GO:0046872 metal ion binding
GO:0005634 nucleus
GO:0006304 DNA modification
View more products with TdT specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products