TdT | HT-1, HT-4, HT-8, HT-9

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Mouse anti Human TdT:FITC

Product Type
Monoclonal Antibody Panel
Clone
HT-1, HT-4, HT-8, HT-9
Isotype
Cocktail
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
OBT0012 F* 25 µg
Mouse anti Human TdT antibody panel, clones HT-1, HT-4, Ht-8 and HT-9 is a mixture of 4 monoclonal antibodies specific for human terminal deoxynucleotidyl transferase (TdT). TdT is a DNA polymerase responsible for the catalysis of non-reversible addition of deoxynucleotides to the 3' end hydroxy groups of DNA. TdT levels are enhanced in all forms of acute lymphoblastic leukaemia (ALL) and in a significant number of chronic lymphoblastic leukaemia cases (CML). TdT is not expressed in the majority of myeloid leukaemias, non-Hodgkins lymphomas or mature lymphoid leukaemias.

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Approx. Protein Concentrations
IgG concentration 50.0 ug/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
Please see label for expiry date.

More Information

UniProt
P04053 Related reagents
Entrez Gene
DNTT Related reagents
GO Terms
GO:0006260 DNA replication
GO:0003677 DNA binding
GO:0003887 DNA-directed DNA polymerase activity
GO:0003912 DNA nucleotidylexotransferase activity
GO:0046872 metal ion binding
GO:0005634 nucleus
GO:0006304 DNA modification
Regulatory
For research purposes only

Applications of TdT

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 Neat
  1. 1 Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F

Product Specific References

References for TdT

  1. Wolgast, L.R. et al. (2011) Spectrin isoforms: differential expression in normal hematopoiesis and alterations in neoplastic bone marrow disorders.
    Am J Clin Pathol. 2011 Aug;136(2):300-8.