TDP43 antibody | 2E2-D3
Mouse anti Human TDP43 antibody, clone 2E2-D3 recognizes human TAR DNA-binding protein 43, also known as TDP43. TPD43 is a 414 amino acid ~45 kDa regulator of transcription and splicing.
Two isoforms of TDP43 generated by alternative splicing have been described. Mouse anti Human TDP43 antibody, clone 2E2-D3 binds to the canonical isoform 1. Binding to the truncated isoform 2 which lacks a significant portion of the immunogen sequence used to generate clone 2E2-D3 has not been established.
Mutations in the TDP43 gene can result in the development of Amyotrophic lateral sclerosis 10 (ALS10), A neurodegenerative disorder affecting upper motor neurons in the brain and lower motor neurons in the brainstem and spinal cord, resulting in fatal paralysis (Yokoseki et al. 2008).
Two isoforms of TDP43 generated by alternative splicing have been described. Mouse anti Human TDP43 antibody, clone 2E2-D3 binds to the canonical isoform 1. Binding to the truncated isoform 2 which lacks a significant portion of the immunogen sequence used to generate clone 2E2-D3 has not been established.
Mutations in the TDP43 gene can result in the development of Amyotrophic lateral sclerosis 10 (ALS10), A neurodegenerative disorder affecting upper motor neurons in the brain and lower motor neurons in the brainstem and spinal cord, resulting in fatal paralysis (Yokoseki et al. 2008).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Immunogen
- Recombinant protein corresponding to aa 1-261 of human TDP43
- Approx. Protein Concentrations
- Ig concentration 0.5 mg/ml
- Fusion Partners
- Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
Storage Information
- Storage
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- Q13148 Related reagents
- Entrez Gene
- TARDBP Related reagents
- Regulatory
- For research purposes only
Applications of TDP43 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence | 0.1 | 10 ug/ml | |
| Immunohistology - Paraffin | 0.1 | 10 ug/ml | |
| Western Blotting | 0.1 | 10 ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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