TDP43 antibody | 2E2-D3
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WB P IF ResetMouse anti Human TDP43:Preservative Free
- Product Type
- Monoclonal Antibody
- Clone
- 2E2-D3
- Isotype
- IgG1
- Specificity
- TDP43
Antibody Quality and Performance Guarantee
| Mouse anti Human TDP43 antibody, clone 2E2-D3 recognizes human TAR DNA-binding protein 43, also known as TDP43. TPD43 is a 414 amino acid ~45 kDa regulator of transcription and splicing. Two isoforms of TDP43 generated by alternative splicing have been described. Mouse anti Human TDP43 antibody, clone 2E2-D3 binds to the canonical isoform 1 recognizing a region between aa205 and 226 as indicated by epitope mapping studies (Zhang et al. 2008). Binding to the truncated isoform 2 appears likely from results of the epitope mapping studies.. Mutations in the TDP43 gene can result in the development of Amyotrophic lateral sclerosis 10 (ALS10), A neurodegenerative disorder affecting upper motor neurons in the brain and lower motor neurons in the brainstem and spinal cord, resulting in fatal paralysis (Yokoseki et al. 2008). |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Immunogen
- Recombinant protein corresponding to aa 1-261 of human TDP43
- Approx. Protein Concentrations
- Ig concentration 0.5 mg/ml
- Fusion Partners
- Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence |  |
0.1 | 10 ug/ml |
| Immunohistology - Paraffin | |
0.1 | 10 ug/ml |
| Western Blotting | |
0.1 | 10 ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
References for TDP43 antibody
-
Zhang, H.X. et al. (2008) Epitope mapping of 2E2-D3, a monoclonal antibody directed against human TDP-43.
Neurosci Lett. 434 (2): 170-4. -
Ling, H. et al. (2014) TDP-43 pathology is present in most post-encephalitic parkinsonism brains.
Neuropathol Appl Neurobiol. 40 (5): 654-7.
- Synonyms
- TARDBP
- RRID
- AB_2255740
- UniProt
- Q13148
- Entrez Gene
- TARDBP
MCA5071Z
170815
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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