TCF4 antibody | AB01/4A12-6-6
Mouse anti TCF4
TCF4 antibody recognizes transcription factor 4, also known as transcription factor 4, SL3-3 enhancer factor 2, class B basic helix-loop-helix protein 19 or immunoglobulin transcription factor 2.
- Product Type
- PrecisionAb™ Monoclonal
- Clone
- AB01/4A12-6-6
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00631 | WB | 100 µl |
Mouse anti Human TCF4 antibody recognizes TCF4, also known as transcription factor 4, SL3-3 enhancer factor 2, class B basic helix-loop-helix protein 19 or immunoglobulin transcription factor 2.
TCF4 encodes transcription factor 4, a basic helix-loop-helix transcription factor. The encoded protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. TCF4 is broadly expressed, and may play an important role in nervous system development. Defects in TCF4 are a cause of Pitt-Hopkins syndrome. Multiple alternatively spliced transcript variants that encode different proteins have been described (provided by RefSeq, Aug 2011).
Mouse anti Human TCF4 antibody detects a band of 55 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
TCF4 encodes transcription factor 4, a basic helix-loop-helix transcription factor. The encoded protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. TCF4 is broadly expressed, and may play an important role in nervous system development. Defects in TCF4 are a cause of Pitt-Hopkins syndrome. Multiple alternatively spliced transcript variants that encode different proteins have been described (provided by RefSeq, Aug 2011).
Mouse anti Human TCF4 antibody detects a band of 55 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti TCF4 antibody detects a band of approximately 55 kDa in Jurkat cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- E. coli-derived recombinant human TCF4 (aa 1-365)
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Guarantee
- As supplied, 12 months from date of despatch
More Information
- UniProt
- P15884 Related reagents
- Entrez Gene
- TCF4 Related reagents
- GO Terms
- GO:0000978 RNA polymerase II core promoter proximal region sequence-specific DNA binding
- GO:0001011 sequence-specific DNA binding RNA polymerase recruiting transcription factor activity
- GO:0001077 RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription
- GO:0001087 TFIIB-class binding transcription factor activity
- GO:0001093 TFIIB-class transcription factor binding
- GO:0005667 transcription factor complex
- GO:0006367 transcription initiation from RNA polymerase II promoter
- GO:0008013 beta-catenin binding
- GO:0008022 protein C-terminus binding
- GO:0016566 specific transcriptional repressor activity
- GO:0016986 transcription initiation factor activity
- GO:0021769 orbitofrontal cortex development
- GO:0021987 cerebral cortex development
- GO:0046982 protein heterodimerization activity
- GO:0048660 regulation of smooth muscle cell proliferation
- GO:0065004 protein-DNA complex assembly
- GO:0070016 armadillo repeat domain binding
- GO:0070888 E-box binding
- GO:0090090 negative regulation of canonical Wnt receptor signaling pathway
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of TCF4 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
Western Blotting without Trial and Error: Use PrecisionAb™ Antibodies
Watch how PrecisionAb Validated Western Blotting Antibodies are defining a new standard of quality that you can trust to deliver superior results and reproducibility in western blotting.
Contact Information
Online Payment Methods:
- Invoice / Purchase Order
- Credit Card (Maestro, VISA,MasterCard & VISA Electron)
