TCF4 antibody | AB01/4A12-6-6
Mouse anti TCF4
TCF4 antibody recognizes transcription factor 4, also known as transcription factor 4, SL3-3 enhancer factor 2, class B basic helix-loop-helix protein 19 or immunoglobulin transcription factor 2.
TCF4 Antibody with Control Lysate
TCF4 antibody recognizes transcription factor 4, also known as transcription factor 4, SL3-3 enhancer factor 2, class B basic helix-loop-helix protein 19 or immunoglobulin transcription factor 2.
- Product Type
- PrecisionAb™ Monoclonal
- Clone
- AB01/4A12-6-6
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00631 | WB | 100 µl | ||||
| VMA00631KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human TCF4 antibody recognizes TCF4, also known as transcription factor 4, SL3-3 enhancer factor 2, class B basic helix-loop-helix protein 19 or immunoglobulin transcription factor 2.
TCF4 encodes transcription factor 4, a basic helix-loop-helix transcription factor. The encoded protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. TCF4 is broadly expressed, and may play an important role in nervous system development. Defects in TCF4 are a cause of Pitt-Hopkins syndrome. Multiple alternatively spliced transcript variants that encode different proteins have been described (provided by RefSeq, Aug 2011).
Mouse anti Human TCF4 antibody detects a band of 55 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
TCF4 encodes transcription factor 4, a basic helix-loop-helix transcription factor. The encoded protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. TCF4 is broadly expressed, and may play an important role in nervous system development. Defects in TCF4 are a cause of Pitt-Hopkins syndrome. Multiple alternatively spliced transcript variants that encode different proteins have been described (provided by RefSeq, Aug 2011).
Mouse anti Human TCF4 antibody detects a band of 55 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti TCF4 antibody detects a band of approximately 55 kDa in Jurkat cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- E. coli-derived recombinant human TCF4 (aa 1-365)
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg Jurkat lysate lyophilized in RIPA buffer
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
More Information
- UniProt
- P15884 Related reagents
- Entrez Gene
- TCF4 Related reagents
- GO Terms
- GO:0000978 RNA polymerase II core promoter proximal region sequence-specific DNA binding
- GO:0001011 sequence-specific DNA binding RNA polymerase recruiting transcription factor activity
- GO:0001077 RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription
- GO:0001087 TFIIB-class binding transcription factor activity
- GO:0001093 TFIIB-class transcription factor binding
- GO:0005667 transcription factor complex
- GO:0006367 transcription initiation from RNA polymerase II promoter
- GO:0008013 beta-catenin binding
- GO:0008022 protein C-terminus binding
- GO:0016566 specific transcriptional repressor activity
- GO:0016986 transcription initiation factor activity
- GO:0021769 orbitofrontal cortex development
- GO:0021987 cerebral cortex development
- GO:0046982 protein heterodimerization activity
- GO:0048660 regulation of smooth muscle cell proliferation
- GO:0065004 protein-DNA complex assembly
- GO:0070016 armadillo repeat domain binding
- GO:0070888 E-box binding
- GO:0090090 negative regulation of canonical Wnt receptor signaling pathway
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of TCF4 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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