TCF4 antibody | AB01/4A12-6-6
Mouse anti Human TCF4 antibody recognizes TCF4, also known as transcription factor 4, SL3-3 enhancer factor 2, class B basic helix-loop-helix protein 19 or immunoglobulin transcription factor 2.
TCF4 encodes transcription factor 4, a basic helix-loop-helix transcription factor. The encoded protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. TCF4 is broadly expressed, and may play an important role in nervous system development. Defects in TCF4 are a cause of Pitt-Hopkins syndrome. Multiple alternatively spliced transcript variants that encode different proteins have been described (provided by RefSeq, Aug 2011).
Mouse anti Human TCF4 antibody detects a band of 55 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
TCF4 encodes transcription factor 4, a basic helix-loop-helix transcription factor. The encoded protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. TCF4 is broadly expressed, and may play an important role in nervous system development. Defects in TCF4 are a cause of Pitt-Hopkins syndrome. Multiple alternatively spliced transcript variants that encode different proteins have been described (provided by RefSeq, Aug 2011).
Mouse anti Human TCF4 antibody detects a band of 55 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti TCF4 antibody detects a band of approximately 55 kDa in Jurkat cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- E. coli-derived recombinant human TCF4 (aa 1-365)
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P15884
- Entrez Gene
- TCF4
- GO Terms
- GO:0000978 RNA polymerase II core promoter proximal region sequence-specific DNA binding
- GO:0001011 sequence-specific DNA binding RNA polymerase recruiting transcription factor activity
- GO:0001077 RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription
- GO:0001087 TFIIB-class binding transcription factor activity
- GO:0001093 TFIIB-class transcription factor binding
- GO:0005667 transcription factor complex
- GO:0006367 transcription initiation from RNA polymerase II promoter
- GO:0008013 beta-catenin binding
- GO:0008022 protein C-terminus binding
- GO:0016566 specific transcriptional repressor activity
- GO:0016986 transcription initiation factor activity
- GO:0021769 orbitofrontal cortex development
- GO:0021987 cerebral cortex development
- GO:0046982 protein heterodimerization activity
- GO:0048660 regulation of smooth muscle cell proliferation
- GO:0065004 protein-DNA complex assembly
- GO:0070016 armadillo repeat domain binding
- GO:0070888 E-box binding
- GO:0090090 negative regulation of canonical Wnt receptor signaling pathway
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of TCF4 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
