TCF4 antibody | AB01/4A12-6-6

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Mouse anti TCF4

TCF4 antibody recognizes transcription factor 4, also known as transcription factor 4, SL3-3 enhancer factor 2, class B basic helix-loop-helix protein 19 or immunoglobulin transcription factor 2.

TCF4 Antibody with Control Lysate

TCF4 antibody recognizes transcription factor 4, also known as transcription factor 4, SL3-3 enhancer factor 2, class B basic helix-loop-helix protein 19 or immunoglobulin transcription factor 2.

Product Type
PrecisionAb™ Monoclonal
Clone
AB01/4A12-6-6
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00631 WB 100 µl
VMA00631KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human TCF4 antibody recognizes TCF4, also known as transcription factor 4, SL3-3 enhancer factor 2, class B basic helix-loop-helix protein 19 or immunoglobulin transcription factor 2.

TCF4 encodes transcription factor 4, a basic helix-loop-helix transcription factor. The encoded protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. TCF4 is broadly expressed, and may play an important role in nervous system development. Defects in TCF4 are a cause of Pitt-Hopkins syndrome. Multiple alternatively spliced transcript variants that encode different proteins have been described (provided by RefSeq, Aug 2011).

Mouse anti Human TCF4 antibody detects a band of 55 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
E. coli-derived recombinant human TCF4 (aa 1-365)

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P15884 Related reagents
Entrez Gene
TCF4 Related reagents
GO Terms
GO:0000978 RNA polymerase II core promoter proximal region sequence-specific DNA binding
GO:0001011 sequence-specific DNA binding RNA polymerase recruiting transcription factor activity
GO:0001077 RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription
GO:0001087 TFIIB-class binding transcription factor activity
GO:0001093 TFIIB-class transcription factor binding
GO:0005667 transcription factor complex
GO:0006367 transcription initiation from RNA polymerase II promoter
GO:0008013 beta-catenin binding
GO:0008022 protein C-terminus binding
GO:0016566 specific transcriptional repressor activity
GO:0016986 transcription initiation factor activity
GO:0021769 orbitofrontal cortex development
GO:0021987 cerebral cortex development
GO:0046982 protein heterodimerization activity
GO:0048660 regulation of smooth muscle cell proliferation
GO:0065004 protein-DNA complex assembly
GO:0070016 armadillo repeat domain binding
GO:0070888 E-box binding
GO:0090090 negative regulation of canonical Wnt receptor signaling pathway
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of TCF4 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Mouse anti TCF4 antibody detects a band of approximately 55 kDa in Jurkat cell lysates
Western Blotting
Mouse anti TCF4 antibody detects a band of approximately 55 kDa in Jurkat cell lysates
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB