TCF12 antibody | OTI4D6

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Mouse anti TCF12

Product Type
PrecisionAb Monoclonal
Clone
OTI4D6
Isotype
IgG2b
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00184
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human TCF12 antibody recognizes TCF12, also known as DNA-binding protein HTF4, E-box-binding protein, class B basic helix-loop-helix protein 20, helix-loop-helix transcription factor 4 and transcription factor HTF-4.

The protein encoded by the TCF12 gene is a member of the basic helix-loop-helix (bHLH) E-protein family that recognizes the consensus binding site (E-box) CANNTG. This encoded protein is expressed in many tissues, among them skeletal muscle, thymus, B- and T-cells, and may participate in regulating lineage-specific gene expression through the formation of heterodimers with other bHLH E-proteins. Several alternatively spliced transcript variants of TCF12 have been described, but the full-length nature of some of these variants has not been determined (provided by RefSeq, Jul 2008).

Mouse anti Human TCF12 antibody detects a band of 85 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti TCF12 detects a band of approximately 85 kDa in Jurkat cell lysates.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Recombinant protein corresponding to amino acids 1-250 of human TCF12 (NP_996919) produced in E. coli

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
Q99081 Related reagents
Entrez Gene
TCF12 Related reagents
GO Terms
GO:0005515 protein binding
GO:0003677 DNA binding
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0003702 RNA polymerase II transcription factor activity
GO:0005634 nucleus
GO:0006357 regulation of transcription from RNA polymerase II promoter
GO:0006955 immune response
GO:0007517 muscle organ development
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of TCF12 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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