TCF12 antibody | OTI4D6

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Anti TCF12 Antibody, clone OTI4D6 (PrecisionAb™ Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with TCF12 antibody (**VMA00184**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc™ MP with 120 second exposure. Arrow points to TCF12 (molecular weight 85 kDa).

Mouse anti TCF12

TCF12 antibody recognizes transcription factor 12, also known as DNA-binding protein HTF4, E-box-binding protein, class B basic helix-loop-helix protein 20, helix-loop-helix transcription factor 4 or transcription factor HTF-4.

TCF12 Antibody with Control Lysate

Product Type: PrecisionAb™ Monoclonal
Clone: OTI4D6
Isotype: IgG2b
Format: Purified

Product Code	Applications	Pack Size
VMA00184	WB	100 µl
VMA00184KT	WB	2 Westerns
With Lysate		400µg

Summary
Secondary Antibodies

Mouse anti Human TCF12 antibody recognizes TCF12, also known as DNA-binding protein HTF4, E-box-binding protein, class B basic helix-loop-helix protein 20, helix-loop-helix transcription factor 4 and transcription factor HTF-4.

The protein encoded by the TCF12 gene is a member of the basic helix-loop-helix (bHLH) E-protein family that recognizes the consensus binding site (E-box) CANNTG. This encoded protein is expressed in many tissues, among them skeletal muscle, thymus, B- and T-cells, and may participate in regulating lineage-specific gene expression through the formation of heterodimers with other bHLH E-proteins. Several alternatively spliced transcript variants of TCF12 have been described, but the full-length nature of some of these variants has not been determined (provided by RefSeq, Jul 2008).

Mouse anti Human TCF12 antibody detects a band of 85 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species: Human
Western Blotting: Anti TCF12 detects a band of approximately 85 kDa in Jurkat cell lysates.
Product Form: Purified IgG - liquid
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography from ascites.
Preparation: 20μl Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Immunogen: Recombinant protein corresponding to amino acids 1-250 of human TCF12 (NP_996919) produced in E. coli
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition: 400μg Jurkat lysate lyophilized in RIPA buffer.
Lysate Reconstitution: - If using DDT reconstitute the lyophilized lysate with 190μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Storage: Antibody: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life: As supplied, 12 months from date of despatch.
Shelf Life: As supplied, 12 months from date of despatch.

More Information

UniProt: Q99081 Related reagents
Entrez Gene: TCF12 Related reagents
GO Terms: GO:0005515 protein binding; GO:0003677 DNA binding; GO:0003700 sequence-specific DNA binding transcription factor activity; GO:0003702 RNA polymerase II transcription factor activity; GO:0005634 nucleus; GO:0006357 regulation of transcription from RNA polymerase II promoter; GO:0006955 immune response; GO:0007517 muscle organ development
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of TCF12 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB

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