TCF12 antibody | OTI4D6
Mouse anti TCF12
TCF12 antibody recognizes transcription factor 12, also known as DNA-binding protein HTF4, E-box-binding protein, class B basic helix-loop-helix protein 20, helix-loop-helix transcription factor 4 or transcription factor HTF-4.
- Product Type
- PrecisionAb™ Monoclonal
- Clone
- OTI4D6
- Isotype
- IgG2b
- Format
- Purified
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| VMA00184 | WB | 100 µl |
|
Mouse anti Human TCF12 antibody recognizes TCF12, also known as DNA-binding protein HTF4, E-box-binding protein, class B basic helix-loop-helix protein 20, helix-loop-helix transcription factor 4 and transcription factor HTF-4.
The protein encoded by the TCF12 gene is a member of the basic helix-loop-helix (bHLH) E-protein family that recognizes the consensus binding site (E-box) CANNTG. This encoded protein is expressed in many tissues, among them skeletal muscle, thymus, B- and T-cells, and may participate in regulating lineage-specific gene expression through the formation of heterodimers with other bHLH E-proteins. Several alternatively spliced transcript variants of TCF12 have been described, but the full-length nature of some of these variants has not been determined (provided by RefSeq, Jul 2008).
Mouse anti Human TCF12 antibody detects a band of 85 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
The protein encoded by the TCF12 gene is a member of the basic helix-loop-helix (bHLH) E-protein family that recognizes the consensus binding site (E-box) CANNTG. This encoded protein is expressed in many tissues, among them skeletal muscle, thymus, B- and T-cells, and may participate in regulating lineage-specific gene expression through the formation of heterodimers with other bHLH E-proteins. Several alternatively spliced transcript variants of TCF12 have been described, but the full-length nature of some of these variants has not been determined (provided by RefSeq, Jul 2008).
Mouse anti Human TCF12 antibody detects a band of 85 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti TCF12 detects a band of approximately 85 kDa in Jurkat cell lysates.
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography from ascites.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- Recombinant protein corresponding to amino acids 1-250 of human TCF12 (NP_996919) produced in E. coli
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Guarantee
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- Q99081 Related reagents
- Entrez Gene
- TCF12 Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0003677 DNA binding
- GO:0003700 sequence-specific DNA binding transcription factor activity
- GO:0003702 RNA polymerase II transcription factor activity
- GO:0005634 nucleus
- GO:0006357 regulation of transcription from RNA polymerase II promoter
- GO:0006955 immune response
- GO:0007517 muscle organ development
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of TCF12 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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