TCEAL1 antibody | OTI3B7

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Anti TCEAL1 Antibody, clone OTI3B7 (PrecisionAb™ Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with TCEAL1 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 299 second exposure. Arrow points to TCEAL1 (molecular weight 27 kDa)

Mouse anti TCEAL1

TCEAL1 antibody recognizes transcription elongation factor A protein-like 1, also known as TCEA-like protein 1, nuclear phosphoprotein p21/SIIR or transcription elongation factor S-II protein-like 1. This antibody has been extensively validated for western blotting using whole cell lysates.

TCEAL1 Antibody with Control Lysate

Product Type: PrecisionAb™ Monoclonal
Clone: OTI3B7
Isotype: IgG1
Format: Purified

Product Code	Applications	Pack Size
VMA00295	WB	100 µl
VMA00295KT	WB	2 Westerns
With Lysate		400µg

Summary
Secondary Antibodies

Mouse anti Human TCEAL1 antibody recognizes the transcription elongation factor A protein-like 1, also known as TCEA-like protein 1, nuclear phosphoprotein p21/SIIR and transcription elongation factor S-II protein-like 1.

Encoded by the TCEAL1 gene, TCEA-like protein 1 is a member of the transcription elongation factor A (SII)-like (TCEAL) gene family. Members of this family may function as nuclear phosphoproteins that modulate transcription in a promoter context-dependent manner. The encoded protein is similar to transcription elongation factor A/transcription factor SII and contains a zinc finger-like motif as well as a sequence related to the transcription factor SII Pol II-binding region. It may exert its effects via protein-protein interactions with other transcriptional regulators rather than via direct binding of DNA. Multiple family members are located on the X chromosome. Alternative splicing results in multiple transcript variants encoding a single isoform (provided by RefSeq, Jul 2008).

Mouse anti Human TCEAL1 antibody detects a band of 27 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species: Human
Western Blotting: Anti TCEAL1 detects a band of approximately 27 kDa in HEK293 cell lysates
Product Form: Purified IgG - liquid
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography from tissue culture supernatant
Preparation: 20μl Mouse monoclonal antibody purified by affinity chromatography from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Immunogen: Full length recombinant human TCEAL1 (NP_004771) produced in E. coli
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition: 400μg HEK293 lysate lyophilized in RIPA buffer.
Lysate Reconstitution: - If using DDT reconstitute the lyophilized lysate with 190μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Storage: Antibody: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Guarantee: As supplied, 12 months from date of despatch.
Guarantee: As supplied, 12 months from date of despatch.

More Information

UniProt: Q15170 Related reagents
Entrez Gene: TCEAL1 Related reagents
GO Terms: GO:0000122 negative regulation of transcription from RNA polymerase II promoter; GO:0003700 sequence-specific DNA binding transcription factor activity; GO:0003702 RNA polymerase II transcription factor activity; GO:0005634 nucleus
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of TCEAL1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB

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