Targeting Protein For Xklp2 antibody
Mouse anti Targeting Protein For Xklp2
Targeting protein for Xklp2 antibody recognizes the Targeting protein for xklp2, also known as TPX2, P100, targeting protein for Xklp2. The antibody has been extensively validated for western blotting using whole cell lysates.
Targeting Protein For Xklp2 Antibody with Control Lysate
Targeting protein for Xklp2 antibody recognizes the Targeting protein for xklp2, also known as TPX2, P100, targeting protein for Xklp2. The antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Monoclonal
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00302 | WB | 100 µl | ||||
| VMA00302KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human targeting protein for Xklp2 antibody recognizes the targeting protein for Xklp2, also known as TPX2, microtubule-associated protein homolog, TPX2, microtubule-associated, homolog, differentially expressed in cancerous and non-cancerous lung cells 2, differentially expressed in lung cells, hepatocellular carcinoma-associated antigen 519, preferentially expressed in colorectal cancer, protein fls353, restricted expression proliferation associated protein 100 and restricted expression proliferation-associated protein 100.
Mouse anti Human targeting protein for Xklp2 antibody detects a band of 100 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Mouse anti Human targeting protein for Xklp2 antibody detects a band of 100 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti targeting protein for Xklp2 detects a band of approximately 100 kDa in Jurkat cell lysates
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites
- Preparation
- 20μl Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Collection of antigenic peptides from different regions of targeting protein for Xklp2
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg Jurkat lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- Q9ULW0 Related reagents
- Entrez Gene
- TPX2 Related reagents
- GO Terms
- GO:0000922 spindle pole
- GO:0006915 apoptosis
- GO:0005524 ATP binding
- GO:0005525 GTP binding
- GO:0005634 nucleus
- GO:0005737 cytoplasm
- GO:0005874 microtubule
- GO:0007067 mitosis
- GO:0008283 cell proliferation
- GO:0019901 protein kinase binding
- GO:0032147 activation of protein kinase activity
- GO:0051301 cell division
- GO:0060236 regulation of mitotic spindle organization
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of Targeting Protein For Xklp2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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