Synaptophysin antibody

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Mouse anti Synaptophysin

Synaptophysin antibody recognizes the synaptophysin protein, also known as major synaptic vesicle protein p38. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgM
Format
Purified
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00073 WB 100 µl
Mouse anti Human synaptophysin antibody recognizes synaptophysin, also known as major synaptic vesicle protein p38.

Synaptophysin encodes an integral membrane protein of small synaptic vesicles in brain and endocrine cells. Synaptophysin also binds cholesterol and is thought to direct targeting of vesicle-associated membrane protein 2 (synaptobrevin) to intracellular compartments. Mutations in synaptophysin are associated with X-linked mental retardation (XLMR) (provided by RefSeq, Aug 2011).

Mouse anti Human synaptophysin antibody detects a band of approximately 37 kDa. The antibody has been extensively validated for western blotting using brain lysates.

Product Details

Target Species
Human
Western Blotting
Anti synaptophysin detects a band of approximately 37 kDa in mouse brain lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgM - liquid
Preparation
Mouse monoclonal antibody purified by gel filtration from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Crude human synaptic immunoprecipitate
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P08247 Related reagents
Entrez Gene
SYP Related reagents
GO Terms
GO:0005509 calcium ion binding
GO:0005215 transporter activity
GO:0015485 cholesterol binding
GO:0016188 synaptic vesicle maturation
GO:0019717 synaptosome
GO:0030054 cell junction
GO:0030285 integral to synaptic vesicle membrane
GO:0048169 regulation of long-term neuronal synaptic plasticity
GO:0048172 regulation of short-term neuronal synaptic plasticity
GO:0048499 synaptic vesicle membrane organization
Acknowledgements
PrecisionAbâ„¢ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Synaptophysin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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