SUPT3H antibody | OTI3D1
Mouse anti SUPT3H
SUPT3H antibody recognizes the transcription initiation protein SPT3 homolog, also known as SPT3-like protein. This antibody has been extensively validated for western blotting using whole cell lysates.
SUPT3H Antibody with Control Lysate
SUPT3H antibody recognizes the transcription initiation protein SPT3 homolog, also known as SPT3-like protein. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Monoclonal
- Clone
- OTI3D1
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00177 | WB | 100 µl | ||||
| VMA00177KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human SUPT3H antibody recognizes SUPT3H, also known as SPT3-like protein and transcription initiation protein SPT3 homolog.
Mouse anti Human SUPT3H antibody detects a band of 34 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Mouse anti Human SUPT3H antibody detects a band of 34 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti SUPT3H detects a band of approximately 34 kDa in Jurkat cell lysates.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography from ascites.
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatography from ascites.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- Full length recombinant human SUPT3H (NP_852001) produced in E. coli
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg Jurkat lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- O75486 Related reagents
- Entrez Gene
- SUPT3H Related reagents
- GO Terms
- GO:0003677 DNA binding
- GO:0003702 RNA polymerase II transcription factor activity
- GO:0003713 transcription coactivator activity
- GO:0006357 regulation of transcription from RNA polymerase II promoter
- GO:0006366 transcription from RNA polymerase II promoter
- GO:0016563 transcription activator activity
- GO:0016578 histone deubiquitination
- GO:0016986 transcription initiation factor activity
- GO:0030914 STAGA complex
- GO:0033276 transcription factor TFTC complex
- GO:0043966 histone H3 acetylation
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of SUPT3H antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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