SUMO-2 antibody

Mouse anti SUMO-2

Product Type
PrecisionAb Monoclonal
Isotype
IgG2b
Format
Purified
Specificity
SUMO-2

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VMA00340
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Mouse anti Human SUMO-2 antibody recognizes the small ubiquitin-related modifier 2 (SUMO-2), also known as SMT3 homolog 2, SMT3 suppressor of mif two 3 homolog 2, sentrin 2, small ubiquitin-related modifier 2, ubiquitin-like protein SMT3A and ubiquitin-like protein SMT3B.

Encoded by the SUMO2 gene, SUMO-2 is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last two amino acids of the carboxy-terminus have been cleaved off. Numerous pseudogenes have been reported for SUMO2. Alternate transcriptional splice variants, encoding different isoforms, have been characterized (provided by RefSeq, Jul 2008).

Mouse anti Human SUMO-2 antibody detects a band of 18 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti SUMO-2 detects a band of approximately 18 kDa in HEK293 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody prepared by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
Recombinant protein corresponding to amino acids 1-95 of human SUMO-2 expressed in E. coli
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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UniProt
P61956
Entrez Gene
SUMO2
GO Terms
GO:0005634 nucleus
GO:0016925 protein sumoylation
GO:0031625 ubiquitin protein ligase binding
GO:0032436 positive regulation of proteasomal ubiquitin-dependent protein catabolic process
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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