SUMO-1 antibody

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Mouse anti SUMO-1

Product Type
PrecisionAb Monoclonal
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00306
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human SUMO-1 antibody recognizes the small ubiquitin-related modifier 1 (SUMO-1), also known as GAP modifying protein 1, SMT3 homolog 3, SMT3 suppressor of mif two 3 homolog 1, sentrin, small ubiquitin-related modifier 1, ubiquitin-homology domain protein PIC1, ubiquitin-like protein SMT3C and ubiquitin-like protein UBL1.

Encoded by the SUMO1 gene, SUMO-1 is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last four amino acids of the carboxy-terminus have been cleaved off. Several pseudogenes have been reported for SUMO1. Alternate transcriptional splice variants encoding different isoforms have been characterized (provided by RefSeq, Jul 2008).

Mouse anti Human SUMO-1 antibody detects a band of 80 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti SUMO-1 detects a band of approximately 80 kDa in HeLa cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Purified recombinant GST-SUMO-1 fusion protein

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
P63165 Related reagents
Entrez Gene
SUMO1 Related reagents
GO Terms
GO:0005643 nuclear pore
GO:0005737 cytoplasm
GO:0006281 DNA repair
GO:0016481 negative regulation of transcription
GO:0016607 nuclear speck
GO:0016925 protein sumoylation
GO:0031334 positive regulation of protein complex assembly
GO:0031625 ubiquitin protein ligase binding
GO:0031965 nuclear membrane
GO:0032436 positive regulation of proteasomal ubiquitin-dependent protein catabolic process
GO:0032880 regulation of protein localization
GO:0043433 negative regulation of transcription factor activity
GO:0060333 interferon-gamma-mediated signaling pathway
GO:0060334 regulation of interferon-gamma-mediated signaling pathway
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of SUMO-1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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