SUMO-1 antibody | 66AT1273.94.49

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Anti SUMO-1 Antibody, clone 66AT1273.94.49 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti SUMO-1 Antibody, clone 66AT1273.94.49 (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti SUMO-1 Antibody, clone 66AT1273.94.49 (PrecisionAb Monoclonal Antibody) thumbnail image 3

Anti SUMO-1 Antibody, clone 66AT1273.94.49 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with SUMO-1 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 67 second exposure. Arrow points to a frequently reported RanGAP-SUMO-1 complex (molecular weight 80 kDa).

Anti SUMO-1 Antibody, clone 66AT1273.94.49 (PrecisionAb Monoclonal Antibody) gallery image 2 — Western blot analysis of SUMO-1 knockout HEK293 and wild type HEK293 (WT) whole cell lysates probed with (A) Mouse anti SUMO-1 antibody (**VMA00306**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 STAR207P) and (B) hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a (A) 300 second and (B) 30 second exposure.

Anti SUMO-1 Antibody, clone 66AT1273.94.49 (PrecisionAb Monoclonal Antibody) gallery image 3 — Normalized signal intensity of SUMO-1 from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.

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Mouse anti SUMO-1

Product Type: PrecisionAb Monoclonal
Clone: 66AT1273.94.49
Isotype: IgG1
Format: Purified
Specificity: SUMO-1

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Product Code Applications Pack Size List Price Your Price Qty

List Price	Your Price
VMA00306 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Mouse anti Human SUMO-1 antibody recognizes the small ubiquitin-related modifier 1 (SUMO-1), also known as GAP modifying protein 1, SMT3 homolog 3, SMT3 suppressor of mif two 3 homolog 1, sentrin, small ubiquitin-related modifier 1, ubiquitin-homology domain protein PIC1, ubiquitin-like protein SMT3C and ubiquitin-like protein UBL1.

Encoded by the SUMO1 gene, SUMO-1 is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last four amino acids of the carboxy-terminus have been cleaved off. Several pseudogenes have been reported for SUMO1. Alternate transcriptional splice variants encoding different isoforms have been characterized (provided by RefSeq, Jul 2008).

Mouse anti Human SUMO-1 antibody detects a band of 80 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti SUMO-1 detects a band of approximately 80 kDa in HeLa cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody prepared by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: Purified recombinant GST-SUMO-1 fusion protein
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml.
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Rat

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: P63165
Entrez Gene: SUMO1
GO Terms: GO:0005643 nuclear pore; GO:0005737 cytoplasm; GO:0006281 DNA repair; GO:0016481 negative regulation of transcription; GO:0016607 nuclear speck; GO:0016925 protein sumoylation; GO:0031334 positive regulation of protein complex assembly; GO:0031625 ubiquitin protein ligase binding; GO:0031965 nuclear membrane; View More GO Terms; GO:0032436 positive regulation of proteasomal ubiquitin-dependent protein catabolic process; GO:0032880 regulation of protein localization; GO:0043433 negative regulation of transcription factor activity; GO:0060333 interferon-gamma-mediated signaling pathway; GO:0060334 regulation of interferon-gamma-mediated signaling pathway