STUB1 antibody

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Human anti STUB1

STUB1 antibody recognizes STUB1, also known as E3 ubiquitin-protein ligase CHIP, CLL-associated antigen KW-8, STIP1 homology and U-box containing protein 1 or heat shock protein A binding protein 2.

STUB1 Antibody with Control Lysate

STUB1 antibody recognizes STUB1, also known as E3 ubiquitin-protein ligase CHIP, CLL-associated antigen KW-8, STIP1 homology and U-box containing protein 1 or heat shock protein A binding protein 2.

Product Type
PrecisionAb™ Monoclonal
Isotype
HuCAL Fab bivalent
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00607 WB 100 µl
VMA00607KT WB 2 Westerns
With Lysate 400µg
Human anti Human STUB1 antibody recognizes STUB1, also known as E3 ubiquitin-protein ligase CHIP, CLL-associated antigen KW-8, STIP1 homology and U-box containing protein 1 or heat shock protein A binding protein 2.

This gene encodes a protein containing tetratricopeptide repeat and a U-box that functions as a ubiquitin ligase/cochaperone. The encoded protein binds to and ubiquitinates shock cognate 71 kDa protein (Hspa8) and DNA polymerase beta (Polb), among other targets. Mutations in this gene cause spinocerebellar ataxia, autosomal recessive 16. Alternative splicing results in multiple transcript variants. There is a pseudogene for this gene on chromosome 2 (provided by RefSeq, Jun 2014).

Human anti Human STUB1 antibody detects a band of 35 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Human anti STUB1 antibody detects a band of approximately 35 kDa in MCF7 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Human monoclonal antibody purified by metal chelate affinity chromatography from E. coli extract
Preparation
20μl Human monoclonal antibody purified by metal chelate affinity chromatography from E. coli extract
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
E. coli-derived recombinant human STUB1 (aa 1 - 303)
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg MCF7 lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
Q9UNE7 Related reagents
Entrez Gene
STUB1 Related reagents
GO Terms
GO:0000151 ubiquitin ligase complex
GO:0000209 protein polyubiquitination
GO:0030674 protein binding, bridging
GO:0005737 cytoplasm
GO:0006515 misfolded or incompletely synthesized protein catabolic process
GO:0051787 misfolded protein binding
GO:0051879 Hsp90 protein binding
GO:0019900 kinase binding
GO:0030544 Hsp70 protein binding
GO:0030579 ubiquitin-dependent SMAD protein catabolic process
GO:0030911 TPR domain binding
GO:0031371 ubiquitin conjugating enzyme complex
GO:0031398 positive regulation of protein ubiquitination
GO:0031943 regulation of glucocorticoid metabolic process
GO:0032436 positive regulation of proteasomal ubiquitin-dependent protein catabolic process
GO:0034450 ubiquitin-ubiquitin ligase activity
GO:0042405 nuclear inclusion body
GO:0042803 protein homodimerization activity
GO:0043161 proteasomal ubiquitin-dependent protein catabolic process
GO:0046332 SMAD binding
GO:0051604 protein maturation
GO:0051865 protein autoubiquitination
GO:0071218 cellular response to misfolded protein
GO:0090035 positive regulation of cellular chaperone-mediated protein complex assembly
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of STUB1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Human IgG F(ab')2:HRP STAR126P 0.5 ml E WB
Goat anti Human IgG F(ab')2:HRP STAR126P 0.5 ml E WB

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