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STUB1 antibody

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Anti STUB1 Antibody (PrecisionAb Monoclonal Antibody) thumbnail image 1
Anti STUB1 Antibody (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with STUB1 antibody followed by detection with Goat anti Human IgG F(ab')2:HRP (1/2,500, STAR126) and visualized on the ChemiDoc MP with 30 second exposure. Arrow points to STUB1 (molecular weight 35 kDa).

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WB

Human anti STUB1

Product Type
PrecisionAb Monoclonal
Isotype
HuCAL Fab bivalent
Format
Purified
Specificity
STUB1

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Product Code Applications Pack Size List Price Your Price Qty
VMA00607
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 100 µl
List Price Your Price

Human anti Human STUB1 antibody recognizes STUB1, also known as E3 ubiquitin-protein ligase CHIP, CLL-associated antigen KW-8, STIP1 homology and U-box containing protein 1 or heat shock protein A binding protein 2.

This gene encodes a protein containing tetratricopeptide repeat and a U-box that functions as a ubiquitin ligase/cochaperone. The encoded protein binds to and ubiquitinates shock cognate 71 kDa protein (Hspa8) and DNA polymerase beta (Polb), among other targets. Mutations in this gene cause spinocerebellar ataxia, autosomal recessive 16. Alternative splicing results in multiple transcript variants. There is a pseudogene for this gene on chromosome 2 (provided by RefSeq, Jun 2014).

Human anti Human STUB1 antibody detects a band of 35 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Human anti STUB1 antibody detects a band of approximately 35 kDa in MCF7 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Human monoclonal antibody purified by metal chelate affinity chromatography from E. coli extract.
Source
E.coli
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
E. coli-derived recombinant human STUB1 (aa 1 - 303)
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the protein. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Human IgG F(ab')2:HRP STAR126P E WB 0.5 ml
List Price Your Price
Description Goat anti Human IgG F(ab')2:HRP

UniProt
Q9UNE7
Entrez Gene
STUB1
GO Terms
GO:0000151 ubiquitin ligase complex
GO:0000209 protein polyubiquitination
GO:0030674 protein binding, bridging
GO:0005737 cytoplasm
GO:0006515 misfolded or incompletely synthesized protein catabolic process
GO:0051787 misfolded protein binding
GO:0051879 Hsp90 protein binding
GO:0019900 kinase binding
GO:0030544 Hsp70 protein binding
GO:0030579 ubiquitin-dependent SMAD protein catabolic process
GO:0030911 TPR domain binding
GO:0031371 ubiquitin conjugating enzyme complex
GO:0031398 positive regulation of protein ubiquitination
GO:0031943 regulation of glucocorticoid metabolic process
GO:0032436 positive regulation of proteasomal ubiquitin-dependent protein catabolic process
GO:0034450 ubiquitin-ubiquitin ligase activity
GO:0042405 nuclear inclusion body
GO:0042803 protein homodimerization activity
GO:0043161 proteasomal ubiquitin-dependent protein catabolic process
GO:0046332 SMAD binding
GO:0051604 protein maturation
GO:0051865 protein autoubiquitination
GO:0071218 cellular response to misfolded protein
GO:0090035 positive regulation of cellular chaperone-mediated protein complex assembly

VMA00607

170508

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