STK11 antibody | 11F3

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Mouse anti STK11

Product Type
PrecisionAb Monoclonal
Clone
11F3
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00800
Datasheet
WB
SDS
100 µl loader

Mouse anti STK11 antibodyrecognizes serine/threonine-protein kinase 11, also known as liver kinase B1 (LKB1) and renal carcinoma antigen NY-REN-19.

The ubiquitously-expressed protein STK11 is a serine/threonine kinase, acting as a "master kinase" to activate several downstream kinases. STK11 has a downstream effect on various signalling pathways including Wnt, TGF-beta and mTOR, and is important for cell survival, polarity, and energy homeostasis. Although a significant amount is known about the downstream processes controlled by STK11, less is known about the upstream processes that activate it (Dogliotti et al. 2017). The gene encoding STK11 acts as a tumor suppressor through inhibition of the mTOR pathway, but also functions as an oncogene (Pécuchet et al. 2017). About a third of lung cancer tumors have inactive or absent STK11 protein, and STK11 deficiency in patients with lung cancer is associated with increased rates of metastasis and decreased survival (Jin et al. 2018).

Product Details

Target Species
Human
Western Blotting
Mouse anti STK11 antibody detects a band of approximately 53 kDa in K562 cell lysates
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-433 of human STK11
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
Q15831
Entrez Gene
STK11
GO Terms
GO:0000287 magnesium ion binding
GO:0005829 cytosol
GO:0004674 protein serine/threonine kinase activity
GO:0005524 ATP binding
GO:0005634 nucleus
GO:0006112 energy reserve metabolic process
GO:0006468 protein phosphorylation
GO:0007050 cell cycle arrest
GO:0008286 insulin receptor signaling pathway
GO:0030511 positive regulation of transforming growth factor beta receptor signaling pathway
GO:0042304 regulation of fatty acid biosynthetic process
GO:0043276 anoikis
GO:0046320 regulation of fatty acid oxidation
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of STK11 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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