STK11 antibody | 11F3
Mouse anti STK11 antibodyrecognizes serine/threonine-protein kinase 11, also known as liver kinase B1 (LKB1) and renal carcinoma antigen NY-REN-19.
The ubiquitously-expressed protein STK11 is a serine/threonine kinase, acting as a "master kinase" to activate several downstream kinases. STK11 has a downstream effect on various signalling pathways including Wnt, TGF-beta and mTOR, and is important for cell survival, polarity, and energy homeostasis. Although a significant amount is known about the downstream processes controlled by STK11, less is known about the upstream processes that activate it (Dogliotti et al. 2017). The gene encoding STK11 acts as a tumor suppressor through inhibition of the mTOR pathway, but also functions as an oncogene (Pécuchet et al. 2017). About a third of lung cancer tumors have inactive or absent STK11 protein, and STK11 deficiency in patients with lung cancer is associated with increased rates of metastasis and decreased survival (Jin et al. 2018).
The ubiquitously-expressed protein STK11 is a serine/threonine kinase, acting as a "master kinase" to activate several downstream kinases. STK11 has a downstream effect on various signalling pathways including Wnt, TGF-beta and mTOR, and is important for cell survival, polarity, and energy homeostasis. Although a significant amount is known about the downstream processes controlled by STK11, less is known about the upstream processes that activate it (Dogliotti et al. 2017). The gene encoding STK11 acts as a tumor suppressor through inhibition of the mTOR pathway, but also functions as an oncogene (Pécuchet et al. 2017). About a third of lung cancer tumors have inactive or absent STK11 protein, and STK11 deficiency in patients with lung cancer is associated with increased rates of metastasis and decreased survival (Jin et al. 2018).
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti STK11 antibody detects a band of approximately 53 kDa in K562 cell lysates
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
- Immunogen
- E. coli-derived recombinant protein of amino acids 1-433 of human STK11
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q15831
- Entrez Gene
- STK11
- GO Terms
- GO:0000287 magnesium ion binding
- GO:0005829 cytosol
- GO:0004674 protein serine/threonine kinase activity
- GO:0005524 ATP binding
- GO:0005634 nucleus
- GO:0006112 energy reserve metabolic process
- GO:0006468 protein phosphorylation
- GO:0007050 cell cycle arrest
- GO:0008286 insulin receptor signaling pathway
- GO:0030511 positive regulation of transforming growth factor beta receptor signaling pathway
- GO:0042304 regulation of fatty acid biosynthetic process
- GO:0043276 anoikis
- GO:0046320 regulation of fatty acid oxidation
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of STK11 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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