STIM1 antibody | 5A2

Mouse anti Human STIM1:Preservative Free

Product Type
Monoclonal Antibody
Clone
5A2
Isotype
IgG2a
Specificity
STIM1

Product Code Applications Pack Size List Price Your Price Qty
MCA3475Z
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
IF P * WB 0.1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Human STIM1 antibody, clone 5A2 recognizes human Stromal interaction molecule 1, also known as STIM1. STIM1 is a 663 amino acid ~80 kDa single pass type I transmembrane glycoprotein involved in replenishment of depleted intracellular Ca2+.

Mutations in the STIM1 gene can lead to development of Immunodeficiency 10 (IMD10) characterized by recurrent infections and impaired T-cell responses (Fuchs et al. 2012) or of Myopathy, tubular aggregate, 1 (TAM1), a rare developmental myopathy with regular arrays of membrane tubules on muscle (Böhm et al. 2013).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Immunogen
Recombinant protein corresponding to aa 24-686 of human STIM1
Approx. Protein Concentrations
Ig concentration 0.5 mg/ml
Fusion Partners
Spleen cells from immunized Balb/c mice were fused with cells from the Sp2/0 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence 0.1 10 ug/ml
Immunohistology - Paraffin 1 0.1 10 ug/ml
Western Blotting
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
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References for STIM1 antibody

  1. Varga-Szabo, D. et al. (2008) The calcium sensor STIM1 is an essential mediator of arterial thrombosis and ischemic brain infarction.
    J Exp Med. 205: 1583-91.

Synonyms
Stromal Interaction Molecule 1
RRID
AB_2198015
UniProt
Q13586
Entrez Gene
STIM1
GO Terms
GO:0005509 calcium ion binding
GO:0005887 integral to plasma membrane
GO:0005513 detection of calcium ion
GO:0005874 microtubule
GO:0006816 calcium ion transport
GO:0030168 platelet activation
GO:0030176 integral to endoplasmic reticulum membrane
GO:0032237 activation of store-operated calcium channel activity
GO:0051010 microtubule plus-end binding
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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