STIM1 antibody | 5A2
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IF P WB ResetMouse anti Human STIM1:Preservative Free
- Product Type
- Monoclonal Antibody
- Clone
- 5A2
- Isotype
- IgG2a
- Specificity
- STIM1
Antibody Quality and 
Unrivaled 
| Mouse anti Human STIM1 antibody, clone 5A2 recognizes human Stromal interaction molecule 1, also known as STIM1. STIM1 is a 663 amino acid ~80 kDa single pass type I transmembrane glycoprotein involved in replenishment of depleted intracellular Ca2+. Mutations in the STIM1 gene can lead to development of Immunodeficiency 10 (IMD10) characterized by recurrent infections and impaired T-cell responses (Fuchs et al. 2012) or of Myopathy, tubular aggregate, 1 (TAM1), a rare developmental myopathy with regular arrays of membrane tubules on muscle (Böhm et al. 2013). |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Immunogen
- Recombinant protein corresponding to aa 24-686 of human STIM1
- Approx. Protein Concentrations
- Ig concentration 0.5 mg/ml
- Fusion Partners
- Spleen cells from immunized Balb/c mice were fused with cells from the Sp2/0 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence |  |
0.1 | 10 ug/ml |
| Immunohistology - Paraffin 1 | |
0.1 | 10 ug/ml |
| Western Blotting | |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
References for STIM1 antibody
-
Varga-Szabo, D. et al. (2008) The calcium sensor STIM1 is an essential mediator of arterial thrombosis and ischemic brain infarction.
J Exp Med. 205: 1583-91.
- Synonyms
- Stromal Interaction Molecule 1
- RRID
- AB_2198015
- UniProt
- Q13586
- Entrez Gene
- STIM1
- GO Terms
- GO:0005509 calcium ion binding
- GO:0005887 integral to plasma membrane
- GO:0005513 detection of calcium ion
- GO:0005874 microtubule
- GO:0006816 calcium ion transport
- GO:0030168 platelet activation
- GO:0030176 integral to endoplasmic reticulum membrane
- GO:0032237 activation of store-operated calcium channel activity
- GO:0051010 microtubule plus-end binding
- View More GO Terms
View more products with STIM1 specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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