STAT5A antibody | OTI6D6

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Mouse anti STAT5A

STAT5A antibody recognizes the signal transducer and activator of transcription 5A. The antibody has been validated for western blotting using whole cell lysates.

STAT5A Antibody with Control Lysate

STAT5A antibody recognizes the signal transducer and activator of transcription 5A. The antibody has been validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Clone
OTI6D6
Isotype
IgG2b
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00712 WB 100 µl
VMA00712KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human STAT5A antibody recognizes the signal transducer and activator of transcription 5A

Encoded by STAT5A gene, signal transducer and activator of transcription 5A is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of STAT5A is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of STAT5A in cells (provided by RefSeq, Jul 2008).

Mouse anti Human STAT5A antibody detects a band of 90 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Product Form
Purified IgG - Liquid
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein A from ascites
Preparation
20μl Mouse monoclonal antibody affinity purified on Protein A from ascites
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
<50% Glycerol
Preservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
<50% Glycerol
Immunogen
Full length recombinant human STAT5A produced in HEK293T cells

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P42229 Related reagents
Entrez Gene
STAT5A Related reagents
GO Terms
GO:0005515 protein binding
GO:0000255 allantoin metabolic process
GO:0005509 calcium ion binding
GO:0003677 DNA binding
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0005829 cytosol
GO:0005654 nucleoplasm
GO:0006101 citrate metabolic process
GO:0006103 2-oxoglutarate metabolic process
GO:0006105 succinate metabolic process
GO:0006107 oxaloacetate metabolic process
GO:0006549 isoleucine metabolic process
GO:0006573 valine metabolic process
GO:0006600 creatine metabolic process
GO:0006631 fatty acid metabolic process
GO:0019530 taurine metabolic process
GO:0046449 creatinine metabolic process
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of STAT5A antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Mouse anti STAT5A detects a band of approximately 90 kDa in K562 cell lysates
Western Blotting
Mouse anti STAT5A detects a band of approximately 90 kDa in K562 cell lysates
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB