Rabbit anti Human STAT3 (pTyr705) antibody recognizes signal transducer and activator of transcription 3 (STAT3), also known as acute-phase response factor (APRF), when phosphorylated at tyrosine 705. STAT3 is a transcription factor, affecting the expression of over a thousand gene products, is an important regulator of cell proliferation and survival, and plays a role in autophagy (You et al. 2015). Activation of STAT3 mostly occurs through phosphorylation of Tyr705, leading to translocation of STAT3 dimers to the nucleus. STAT3 can regulate both tumor suppressor genes and oncogenes, and is able to regulate tumor microenvironments (Galoczova et al. 2018). Constitutive activation of STAT3 has been identified in tumor cells and tumor-infiltrated immune cells, and activated STAT3 triggers tumor progression by directly regulating expression of oncogenic genes (Wang et al. 2018).
- Target Species
- Western Blotting
- Rabbit anti STAT3 (pTyr705) antibody detects a band of approximately 84 kDa in AA431 cell lysates
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
||Expected from Sequence
||Expected from Sequence
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from animal origin
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
1% Bovine Serum Albumin
- A phospho-peptide corresponding to Human STAT3 (pTyr705)
- Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
negative regulation of transcription from RNA polymerase II promoter
calcium ion binding
eye photoreceptor cell differentiation
sequence-specific DNA binding transcription factor activity
protein import into nucleus
signal transducer activity
cellular component movement
transcription factor binding
transcription activator activity
protein kinase binding
response to estradiol stimulus
interspecies interaction between organisms
protein dimerization activity
nerve growth factor receptor signaling pathway
JAK-STAT cascade involved in growth hormone signaling pathway
interleukin-6-mediated signaling pathway
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of STAT3 (pTyr705) antibody
|Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
How to Use the Spectraviewer?
Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
Select the lasers and filters you wish to include
Select combined or multi-laser view to visualize the spectra