STAT3 (pTyr705) antibody | RM261
Rabbit anti Human STAT3 (pTyr705) antibody recognizes signal transducer and activator of transcription 3 (STAT3), also known as acute-phase response factor (APRF), when phosphorylated at tyrosine 705. STAT3 is a transcription factor, affecting the expression of over a thousand gene products, is an important regulator of cell proliferation and survival, and plays a role in autophagy (You et al. 2015). Activation of STAT3 mostly occurs through phosphorylation of Tyr705, leading to translocation of STAT3 dimers to the nucleus. STAT3 can regulate both tumor suppressor genes and oncogenes, and is able to regulate tumor microenvironments (Galoczova et al. 2018). Constitutive activation of STAT3 has been identified in tumor cells and tumor-infiltrated immune cells, and activated STAT3 triggers tumor progression by directly regulating expression of oncogenic genes (Wang et al. 2018).
Product Details
- Target Species
- Human
- Western Blotting
- Rabbit anti STAT3 (pTyr705) antibody detects a band of approximately 84 kDa in AA431 cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Expected from Sequence Rat Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- A phospho-peptide corresponding to Human STAT3 (pTyr705)
Storage Information
- Storage
- Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P40763
- Entrez Gene
- STAT3
- GO Terms
- GO:0005886 plasma membrane
- GO:0000122 negative regulation of transcription from RNA polymerase II promoter
- GO:0005509 calcium ion binding
- GO:0001659 temperature homeostasis
- GO:0001754 eye photoreceptor cell differentiation
- GO:0003700 sequence-specific DNA binding transcription factor activity
- GO:0006606 protein import into nucleus
- GO:0005829 cytosol
- GO:0004871 signal transducer activity
- GO:0005634 nucleus
- GO:0006928 cellular component movement
- GO:0008134 transcription factor binding
- GO:0016563 transcription activator activity
- GO:0019901 protein kinase binding
- GO:0019953 sexual reproduction
- GO:0032355 response to estradiol stimulus
- GO:0042593 glucose homeostasis
- GO:0042755 eating behavior
- GO:0044419 interspecies interaction between organisms
- GO:0046983 protein dimerization activity
- GO:0048011 nerve growth factor receptor signaling pathway
- GO:0060397 JAK-STAT cascade involved in growth hormone signaling pathway
- GO:0070102 interleukin-6-mediated signaling pathway
- Regulatory
- For research purposes only
Applications of STAT3 (pTyr705) antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin | 1/1000 | 1/10,000 | |
| Western Blotting | 1/1000 | 1/2000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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