STAT3 (pTyr705) antibody | RM261

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Rabbit anti STAT3 (pTyr705)

Product Type
PrecisionAb Monoclonal
Clone
RM261
Isotype
IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00899
Datasheet
P WB
SDS
100 µl loader

Rabbit anti Human STAT3 (pTyr705) antibody recognizes signal transducer and activator of transcription 3 (STAT3), also known as acute-phase response factor (APRF), when phosphorylated at tyrosine 705. STAT3 is a transcription factor, affecting the expression of over a thousand gene products, is an important regulator of cell proliferation and survival, and plays a role in autophagy (You et al. 2015). Activation of STAT3 mostly occurs through phosphorylation of Tyr705, leading to translocation of STAT3 dimers to the nucleus. STAT3 can regulate both tumor suppressor genes and oncogenes, and is able to regulate tumor microenvironments (Galoczova et al. 2018). Constitutive activation of STAT3 has been identified in tumor cells and tumor-infiltrated immune cells, and activated STAT3 triggers tumor progression by directly regulating expression of oncogenic genes (Wang et al. 2018).

Product Details

Target Species
Human
Western Blotting
Rabbit anti STAT3 (pTyr705) antibody detects a band of approximately 84 kDa in AA431 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
Rat Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from animal origin
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
50% Glycerol
Immunogen
A phospho-peptide corresponding to Human STAT3 (pTyr705)

Storage Information

Storage
Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P40763
Entrez Gene
STAT3
GO Terms
GO:0005886 plasma membrane
GO:0000122 negative regulation of transcription from RNA polymerase II promoter
GO:0005509 calcium ion binding
GO:0001659 temperature homeostasis
GO:0001754 eye photoreceptor cell differentiation
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0006606 protein import into nucleus
GO:0005829 cytosol
GO:0004871 signal transducer activity
GO:0005634 nucleus
GO:0006928 cellular component movement
GO:0008134 transcription factor binding
GO:0016563 transcription activator activity
GO:0019901 protein kinase binding
GO:0019953 sexual reproduction
GO:0032355 response to estradiol stimulus
GO:0042593 glucose homeostasis
GO:0042755 eating behavior
GO:0044419 interspecies interaction between organisms
GO:0046983 protein dimerization activity
GO:0048011 nerve growth factor receptor signaling pathway
GO:0060397 JAK-STAT cascade involved in growth hormone signaling pathway
GO:0070102 interleukin-6-mediated signaling pathway
Regulatory
For research purposes only

Applications of STAT3 (pTyr705) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1/1000 1/10,000
Western Blotting 1/1000 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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