SRSF1 antibody | AB01/3A7

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Mouse anti SRSF1

SRSF1 Antibody with Control Lysate

Product Type
PrecisionAb™ Monoclonal
Clone
AB01/3A7
Isotype
IgG2b
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00538 WB 100 µl
VMA00538KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human SRSF1 antibody recognizes the serine/arginine-rich splicing factor 1, also known as SR splicing factor 1, alternative-splicing factor 1, pre-mRNA-splicing factor SF2 P33 subunit.

The SRSF1 gene encodes a member of the arginine/serine-rich splicing factor protein family, and functions in both constitutive and alternative pre-mRNA splicing. The protein binds to pre-mRNA transcripts and components of the spliceosome, and can either activate or repress splicing depending on the location of the pre-mRNA binding site. The protein's ability to activate splicing is regulated by phosphorylation and interactions with other splicing factor associated proteins. Multiple transcript variants encoding different isoforms have been found for SRSF1. In addition, a pseudogene of SRSF1 has been found on chromosome 13 (provided by RefSeq, Sep 2010).

Mouse anti Human SRSF1 antibody detects a band of 33 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from ascites
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography Protein G from ascites
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
E. coli-derived recombinant human SRSF1 (amino acids 1 - 248)

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
Q07955 Related reagents
Entrez Gene
SRSF1 Related reagents
GO Terms
GO:0000166 nucleotide binding
GO:0005515 protein binding
GO:0003723 RNA binding
GO:0005737 cytoplasm
GO:0005681 spliceosomal complex
GO:0006369 termination of RNA polymerase II transcription
GO:0006376 mRNA splice site selection
GO:0006406 mRNA export from nucleus
GO:0016607 nuclear speck
GO:0031124 mRNA 3'-end processing
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of SRSF1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti SRSF1 detects a band of approximately 33 kDa in LNCaP cell lysates
Western Blotting
Anti SRSF1 detects a band of approximately 33 kDa in LNCaP cell lysates
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB