SRSF1 antibody | AB01/3A7

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Anti SRSF1 Antibody, clone AB01/3A7 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti SRSF1 Antibody, clone AB01/3A7 (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti SRSF1 Antibody, clone AB01/3A7 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with SRSF1 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/5,000, STAR207P) and visualized on the ChemiDoc MP with 21 second exposure. Arrow points to SRSF1 (molecular weight 33 kDa)

Anti SRSF1 Antibody, clone AB01/3A7 (PrecisionAb Monoclonal Antibody) gallery image 2 — Immunoprecipitation of SRSF1 from LNCaP whole cell lysate. The Input lane is 10% input; the SRSF1-IP lane is the immunoprecipitation with the SRSF1 antibody (2.5 µg, **VMA00538**); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG (2.5 µg, MCA691). Western blot analysis was performed using SRSF1 antibody (1/1,000, **VMA00538**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/5,000, 1705047) and visualized on the ChemiDoc MP. Arrow points to SRSF1.

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WB IP

Mouse anti SRSF1

Product Type: PrecisionAb Monoclonal
Clone: AB01/3A7
Isotype: IgG2b
Format: Purified
Specificity: SRSF1

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Product Code Applications Pack Size List Price Your Price Qty

List Price	Your Price
VMA00538 Datasheet Datasheet SDS SDS	IP WB	100 µl		Log in	Get Quote
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Mouse anti Human SRSF1 antibody recognizes the serine/arginine-rich splicing factor 1, also known as SR splicing factor 1, alternative-splicing factor 1, pre-mRNA-splicing factor SF2 P33 subunit.

The SRSF1 gene encodes a member of the arginine/serine-rich splicing factor protein family, and functions in both constitutive and alternative pre-mRNA splicing. The protein binds to pre-mRNA transcripts and components of the spliceosome, and can either activate or repress splicing depending on the location of the pre-mRNA binding site. The protein's ability to activate splicing is regulated by phosphorylation and interactions with other splicing factor associated proteins. Multiple transcript variants encoding different isoforms have been found for SRSF1. In addition, a pseudogene of SRSF1 has been found on chromosome 13 (provided by RefSeq, Sep 2010).

Mouse anti Human SRSF1 antibody detects a band of 33 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti SRSF1 detects a band of approximately 33 kDa in LNCaP cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography on Protein G from ascites.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: E. coli-derived recombinant human SRSF1 (amino acids 1 - 248)
Approx. Protein Concentrations: IgG concentration 2.0 mg/ml.
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse
Rat

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunoprecipitation
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: Q07955
Entrez Gene: SRSF1
GO Terms: GO:0000166 nucleotide binding; GO:0005515 protein binding; GO:0003723 RNA binding; GO:0005737 cytoplasm; GO:0005681 spliceosomal complex; GO:0006369 termination of RNA polymerase II transcription; GO:0006376 mRNA splice site selection; GO:0006406 mRNA export from nucleus; GO:0016607 nuclear speck; View More GO Terms; GO:0031124 mRNA 3'-end processing