SRC antibody | ST03/3E9
Mouse anti SRC antibody recognizes proto-oncogene tyrosine-protein kinase Src, also known as Src1, p60-Src and c-Src.
c-Src is a ubiquitously-expressed cytoplasmic tyrosine kinase, important for regulating diverse cellular functions (Honda et al. 2018). c-Src was the first oncogene to be identified, overexpressed and abnormally activated in cancer cells, and is the cellular counterpart of v-Src, a cancer-causing gene found in the Rous sarcoma virus (Hunter and Sefton 1980). Src functions as a complex molecular switch, which can self-modulate its activity in response to specific signals. This is achieved by a reciprocally regulatory mechanism involving Tyr416 and Tyr527, changing the tertiary structure of Src and controlling access to its active site (Knighton et al. 1991).
c-Src is a ubiquitously-expressed cytoplasmic tyrosine kinase, important for regulating diverse cellular functions (Honda et al. 2018). c-Src was the first oncogene to be identified, overexpressed and abnormally activated in cancer cells, and is the cellular counterpart of v-Src, a cancer-causing gene found in the Rous sarcoma virus (Hunter and Sefton 1980). Src functions as a complex molecular switch, which can self-modulate its activity in response to specific signals. This is achieved by a reciprocally regulatory mechanism involving Tyr416 and Tyr527, changing the tertiary structure of Src and controlling access to its active site (Knighton et al. 1991).
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti SRC detects a band of approximately 58 kDa in A431 cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - Liquid
- Preparation
- Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
- Immunogen
- E. coli-derived recombinant protein of amino acids 351-543 of human SRC
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P12931
- Entrez Gene
- SRC
- GO Terms
- GO:0005829 cytosol
- GO:0004715 non-membrane spanning protein tyrosine kinase activity
- GO:0005070 SH3/SH2 adaptor activity
- GO:0005178 integrin binding
- GO:0005524 ATP binding
- GO:0005901 caveola
- GO:0006468 protein phosphorylation
- GO:0007172 signal complex assembly
- GO:0007173 epidermal growth factor receptor signaling pathway
- GO:0007243 intracellular protein kinase cascade
- GO:0007265 Ras protein signal transduction
- GO:0007411 axon guidance
- GO:0008543 fibroblast growth factor receptor signaling pathway
- GO:0016044 cellular membrane organization
- GO:0030168 platelet activation
- GO:0031295 T cell costimulation
- GO:0033625 positive regulation of integrin activation
- GO:0034329 cell junction assembly
- GO:0042169 SH2 domain binding
- GO:0043114 regulation of vascular permeability
- GO:0044419 interspecies interaction between organisms
- GO:0045124 regulation of bone resorption
- GO:0048011 nerve growth factor receptor signaling pathway
- GO:0050900 leukocyte migration
- GO:0070555 response to interleukin-1
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of SRC antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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