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SRC antibody | ST03/3E9

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Anti SRC Antibody, clone ST03/3E9 (PrecisionAb Monoclonal Antibody) thumbnail image 1
Anti SRC Antibody, clone ST03/3E9 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with SRC antibody (1/1,000, VMA00922) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to SRC (molecular weight 58 kDa)

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WB

Mouse anti SRC

Product Type
PrecisionAb Monoclonal
Clone
ST03/3E9
Isotype
IgG2a
Format
Purified
Specificity
SRC

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Product Code Applications Pack Size List Price Your Price Qty
VMA00922
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 100 µl loader
List Price Your Price
loader

Mouse anti SRC antibody recognizes proto-oncogene tyrosine-protein kinase Src, also known as Src1, p60-Src and c-Src.

c-Src is a ubiquitously-expressed cytoplasmic tyrosine kinase, important for regulating diverse cellular functions (Honda et al. 2018). c-Src was the first oncogene to be identified, overexpressed and abnormally activated in cancer cells, and is the cellular counterpart of v-Src, a cancer-causing gene found in the Rous sarcoma virus (Hunter and Sefton 1980). Src functions as a complex molecular switch, which can self-modulate its activity in response to specific signals. This is achieved by a reciprocally regulatory mechanism involving Tyr416 and Tyr527, changing the tertiary structure of Src and controlling access to its active site (Knighton et al. 1991). 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Mouse anti SRC detects a band of approximately 58 kDa in A431 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 351-543 of human SRC
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):HRP

UniProt
P12931
Entrez Gene
SRC
GO Terms
GO:0005829 cytosol
GO:0004715 non-membrane spanning protein tyrosine kinase activity
GO:0005070 SH3/SH2 adaptor activity
GO:0005178 integrin binding
GO:0005524 ATP binding
GO:0005901 caveola
GO:0006468 protein phosphorylation
GO:0007172 signal complex assembly
GO:0007173 epidermal growth factor receptor signaling pathway
GO:0007243 intracellular protein kinase cascade
GO:0007265 Ras protein signal transduction
GO:0007411 axon guidance
GO:0008543 fibroblast growth factor receptor signaling pathway
GO:0016044 cellular membrane organization
GO:0030168 platelet activation
GO:0031295 T cell costimulation
GO:0033625 positive regulation of integrin activation
GO:0034329 cell junction assembly
GO:0042169 SH2 domain binding
GO:0043114 regulation of vascular permeability
GO:0044419 interspecies interaction between organisms
GO:0045124 regulation of bone resorption
GO:0048011 nerve growth factor receptor signaling pathway
GO:0050900 leukocyte migration
GO:0070555 response to interleukin-1

VMA00922

100005615

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