Mouse anti Human SPI1 antibody, clone 2G1
recognizes human Transcription factor PU.1, also known as SPI1 or 31 kDa-transforming protein. SPI1 is a 270 amino acid ~30 kDa DNA binding protein. Two isoforms of SPI1 generated by alternative splicing have been described, with isoform 2 differing from isoform 1 by a single amino acid insertion at position 16 (UniProt : P17947
). Mouse anti Human SPI1 antibody, clone 2G1 binds to the canonical isoform 1 but binding to isoform 2 has not been evaluated.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Recombinant protein corresponding to aa 1 - 121 of human SPI1.
- Approx. Protein Concentrations
- Ig concentration 0.5 mg/ml
- Fusion Partners
- Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 18 months from date of despatch.
- Entrez Gene
- GO Terms
negative regulation of transcription from RNA polymerase II promoter
sequence-specific DNA binding transcription factor activity
positive regulation of gene-specific transcription
sequence-specific DNA binding
regulation of erythrocyte differentiation
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of SPI1 antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available