Spectrin Beta antibody
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WB F ResetMouse anti Spectrin Beta
- Product Type
- PrecisionAb Monoclonal
- Isotype
- IgG2b
- Format
- Purified
- Specificity
- Spectrin Beta
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| Mouse anti Human spectrin beta antibody recognizes spectrin beta chain, non-erythrocytic 1, also known as beta-G spectrin, beta-spectrin 2, embryonic liver beta-fodrin, epididymis luminal protein 102 or fodrin beta chain. Spectrin is an actin crosslinking and molecular scaffold protein that links the plasma membrane to the actin cytoskeleton, and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. It is composed of two antiparallel dimers of alpha- and beta- subunits. SPTBN1 is one member of a family of beta-spectrin genes. The encoded protein contains an N-terminal actin-binding domain, and 17 spectrin repeats which are involved in dimer formation. Multiple transcript variants encoding different isoforms have been found for SPTBN1 (provided by RefSeq, Jul 2008). Mouse anti Human spectrin beta antibody detects a band of 275 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. |
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- Target Species
- Human
- Western Blotting
- Anti spectrin beta detects a band of approximately 275 kDa in Jurkat cell lysate..
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant.
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3).
- Immunogen
- E. coli derived recombinant protein corresponding to amino acids 2089-2364 of human spectrin beta
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Regulatory
- For research purposes only.
- Guarantee
- 12 months from date of despatch.
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
||
| Western Blotting | |
1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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| List Price | Your Price | ||||||
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| Description | Goat anti Mouse IgG (H/L):HRP | ||||||
- UniProt
- Q01082
- Entrez Gene
- SPTBN1
- GO Terms
- GO:0005886 plasma membrane
- GO:0005515 protein binding
- GO:0003779 actin binding
- GO:0005829 cytosol
- GO:0005200 structural constituent of cytoskeleton
- GO:0005516 calmodulin binding
- GO:0005730 nucleolus
- GO:0007411 axon guidance
- GO:0008091 spectrin
- View More GO Terms
- GO:0031430 M band
- GO:0051693 actin filament capping
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