Spectrin Beta antibody

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Mouse anti Spectrin Beta

Mouse anti-human spectrin beta antibody recognizes spectrin beta chain, non-erythrocytic 1, also known as beta-G spectrin, beta-II spectrin, embryonic liver beta-fodrin, epididymis luminal protein 102 or fodrin beta chain.

Product Type: PrecisionAb™ Monoclonal
Isotype: IgG2b
Format: Purified

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
VMA00514 Datasheet	WB MSDS	100 µl	Log in		Get Quote

Summary
Secondary Antibodies

Mouse anti Human spectrin beta antibody recognizes spectrin beta chain, non-erythrocytic 1, also known as beta-G spectrin, beta-spectrin 2, embryonic liver beta-fodrin, epididymis luminal protein 102 or fodrin beta chain.

Spectrin is an actin crosslinking and molecular scaffold protein that links the plasma membrane to the actin cytoskeleton, and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. It is composed of two antiparallel dimers of alpha- and beta- subunits. SPTBN1 is one member of a family of beta-spectrin genes. The encoded protein contains an N-terminal actin-binding domain, and 17 spectrin repeats which are involved in dimer formation. Multiple transcript variants encoding different isoforms have been found for SPTBN1 (provided by RefSeq, Jul 2008).

Mouse anti Human spectrin beta antibody detects a band of 275 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species: Human
Western Blotting: Anti spectrin beta detects a band of approximately 275 kDa in Jurkat cell lysate
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Immunogen: E. coli derived recombinant protein corresponding to amino acids 2089-2364 of human spectrin beta
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Guarantee: As supplied, 12 months from date of despatch.

More Information

UniProt: Q01082 Related reagents
Entrez Gene: SPTBN1 Related reagents
GO Terms: GO:0005886 plasma membrane; GO:0005515 protein binding; GO:0003779 actin binding; GO:0005829 cytosol; GO:0005200 structural constituent of cytoskeleton; GO:0005516 calmodulin binding; GO:0005730 nucleolus; GO:0007411 axon guidance; GO:0008091 spectrin; GO:0031430 M band; GO:0051693 actin filament capping
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of Spectrin Beta antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in		Get Quote

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