Spectrin Alpha Chain antibody

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Anti Spectrin Alpha Chain Antibody (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with Mouse anti Human spectrin alpha chain antibody (**VMA00079**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 53 second exposure. Arrow points to spectrin alpha chain (molecular weight 285 kDa).

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Mouse anti Spectrin Alpha Chain

Product Type: PrecisionAb Monoclonal
Isotype: IgG2b
Format: Purified
Specificity: Spectrin Alpha Chain

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Mouse anti Human spectrin alpha chain antibody recognizes spectrin alpha chain also known as alpha-II spectrin, fodrin alpha chain spectrin and non-erythroid alpha subunit.

Spectrins are a family of filamentous cytoskeletal proteins that function as essential scaffold proteins that stabilize the plasma membrane and organize intracellular organelles. Spectrins are composed of alpha and beta dimers that associate to form tetramers linked in a head-to-head arrangement. The spectrin alpha chain is specifically expressed in non-erythrocytic cells. The encoded protein has been implicated in other cellular functions including DNA repair and cell cycle regulation. Mutations in the spectrin alpha chain are the cause of early infantile epileptic encephalopathy-5. Alternate splicing results in multiple transcript variants (provided by RefSeq, Sep 2010).

Mouse anti Human spectrin alpha chain antibody detects a band of 285 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti spectrin alpha chain detects a band of approximately 285 kDa in Jurkat cell lysates.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: E. Coli derived recombinant protein, aa 1009-1209 of human spectrin alpha chain
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.