Spectrin Alpha Chain antibody
Mouse anti Spectrin Alpha Chain
Spectrin alpha chain antibody recognizes the spectrin alpha chain, non-erythrocytic 1 protein, also known as alpha-II spectrin, alpha-fodrin, fodrin alpha chain, spectrin or non-erythroid alpha chain. This antibody has been extensively validated for western blotting using whole cell lysates.
Spectrin Alpha Chain Antibody with Control Lysate
Spectrin alpha chain antibody recognizes the spectrin alpha chain, non-erythrocytic 1 protein, also known as alpha-II spectrin, alpha-fodrin, fodrin alpha chain, spectrin or non-erythroid alpha chain. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Monoclonal
- Isotype
- IgG2b
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00079 | WB | 100 µl | ||||
| VMA00079KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human spectrin alpha chain antibody recognizes spectrin alpha chain also known as alpha-II spectrin, fodrin alpha chain spectrin and non-erythroid alpha subunit.
Spectrins are a family of filamentous cytoskeletal proteins that function as essential scaffold proteins that stabilize the plasma membrane and organize intracellular organelles. Spectrins are composed of alpha and beta dimers that associate to form tetramers linked in a head-to-head arrangement. The spectrin alpha chain is specifically expressed in non-erythrocytic cells. The encoded protein has been implicated in other cellular functions including DNA repair and cell cycle regulation. Mutations in the spectrin alpha chain are the cause of early infantile epileptic encephalopathy-5. Alternate splicing results in multiple transcript variants (provided by RefSeq, Sep 2010).
Mouse anti Human spectrin alpha chain antibody detects a band of 285 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Spectrins are a family of filamentous cytoskeletal proteins that function as essential scaffold proteins that stabilize the plasma membrane and organize intracellular organelles. Spectrins are composed of alpha and beta dimers that associate to form tetramers linked in a head-to-head arrangement. The spectrin alpha chain is specifically expressed in non-erythrocytic cells. The encoded protein has been implicated in other cellular functions including DNA repair and cell cycle regulation. Mutations in the spectrin alpha chain are the cause of early infantile epileptic encephalopathy-5. Alternate splicing results in multiple transcript variants (provided by RefSeq, Sep 2010).
Mouse anti Human spectrin alpha chain antibody detects a band of 285 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti spectrin alpha chain detects a band of approximately 285 kDa in Jurkat cell lysates.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- E. Coli derived recombinant protein, aa 1009-1209 of human spectrin alpha chain
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg Jurkat lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
More Information
- UniProt
- Q13813 Related reagents
- Entrez Gene
- SPTAN1 Related reagents
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of Spectrin Alpha Chain antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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