SOCS-3 antibody

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Mouse anti SOCS-3

SOCS-3 antibody recognizes suppressor of cytokine signaling 3, also known as SOCS-3, cytokine-inducible SH2 protein 3, CIS-3, STAT-induced STAT inhibitor 3 or SSI-3. The antibody has been extensively validated for western blotting using whole cell lysates.

SOCS-3 Antibody with Control Lysate

SOCS-3 antibody recognizes suppressor of cytokine signaling 3, also known as SOCS-3, cytokine-inducible SH2 protein 3, CIS-3, STAT-induced STAT inhibitor 3 or SSI-3. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG2b
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00027 WB 100 µl
VMA00027KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human SOCS-3 antibody recognizes the human suppressor of cytokine signaling 3 protein, also known as SOCS-3, cytokine-inducible SH2 protein 3 or STAT-induced STAT inhibitor 3. SOCS-3 is a 225 amino acid member of the suppressor of cytokine signaling family (Starr et al. 1997) containing a single SH2 and a single SOCS box domain. SOCS-3 acts as a negative regulator of janus kinase (JAK) and signal transducer and activators of transcription (STAT) signaling pathways.

SOCS-3 specifically acts as a negative feedback regulator for the STAT3 pathways, by directly binding to the Tyr-759 motif of the IL-6 signal-transducing receptor subunit gp130, thereby preventing the IL-6 activation of STAT3.

The expression of SOCS-3 is inducible by numerous factors, including IL-6, IL-10, toll-like receptor (TLR) agonists and interferon (IFN) gamma, making this protein the focus of research into inflammatory diseases such as arthritis, colitis and Crohn’s disease (Elliott and Johnstone 2004). SOCS-3 expression has also been shown to affect IFN alpha sensitivity in tumor studies (Brender et al. 2001).

Notch signaling plays a critical role in the determination of M1 versus M2 macrophage polarization. RBPJ–mediated notch signaling regulates this polarization through SOCS-3 (Narayana and Balaji 2008, Schroeder and Just 2000).

Mouse anti Human SOCS-3 antibody recognizes human SOCS-3 as a single band of ~25 kDa in MCF7 cell lysates by western blotting under reducing conditions. This antibody also cross reacts with SOCS-3 from murine cell lines.

Product Details

Target Species
Human
Western Blotting
Anti SOCS-3 antibody detects a band of approximately 25 kDa in MCF7 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Full length SOCS-3 protein
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg MCF7 lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
O14543 Related reagents
Entrez Gene
SOCS3 Related reagents
GO Terms
GO:0005829 cytosol
GO:0004860 protein kinase inhibitor activity
GO:0006916 anti-apoptosis
GO:0007259 JAK-STAT cascade
GO:0040008 regulation of growth
GO:0060333 interferon-gamma-mediated signaling pathway
GO:0060334 regulation of interferon-gamma-mediated signaling pathway
GO:0060337 type I interferon-mediated signaling pathway
GO:0060338 regulation of type I interferon-mediated signaling pathway
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of SOCS-3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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