SOCS-3 antibody
Mouse anti SOCS-3
SOCS-3 antibody recognizes suppressor of cytokine signaling 3, also known as SOCS-3, cytokine-inducible SH2 protein 3, CIS-3, STAT-induced STAT inhibitor 3 or SSI-3. The antibody has been extensively validated for western blotting using whole cell lysates.
SOCS-3 Antibody with Control Lysate
SOCS-3 antibody recognizes suppressor of cytokine signaling 3, also known as SOCS-3, cytokine-inducible SH2 protein 3, CIS-3, STAT-induced STAT inhibitor 3 or SSI-3. The antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Monoclonal
- Isotype
- IgG2b
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00027 | WB | 100 µl | ||||
| VMA00027KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human SOCS-3 antibody recognizes the human suppressor of cytokine signaling 3 protein, also known as SOCS-3, cytokine-inducible SH2 protein 3 or STAT-induced STAT inhibitor 3. SOCS-3 is a 225 amino acid member of the suppressor of cytokine signaling family (Starr et al. 1997) containing a single SH2 and a single SOCS box domain. SOCS-3 acts as a negative regulator of janus kinase (JAK) and signal transducer and activators of transcription (STAT) signaling pathways.
SOCS-3 specifically acts as a negative feedback regulator for the STAT3 pathways, by directly binding to the Tyr-759 motif of the IL-6 signal-transducing receptor subunit gp130, thereby preventing the IL-6 activation of STAT3.
The expression of SOCS-3 is inducible by numerous factors, including IL-6, IL-10, toll-like receptor (TLR) agonists and interferon (IFN) gamma, making this protein the focus of research into inflammatory diseases such as arthritis, colitis and Crohn’s disease (Elliott and Johnstone 2004). SOCS-3 expression has also been shown to affect IFN alpha sensitivity in tumor studies (Brender et al. 2001).
Notch signaling plays a critical role in the determination of M1 versus M2 macrophage polarization. RBPJ–mediated notch signaling regulates this polarization through SOCS-3 (Narayana and Balaji 2008, Schroeder and Just 2000).
Mouse anti Human SOCS-3 antibody recognizes human SOCS-3 as a single band of ~25 kDa in MCF7 cell lysates by western blotting under reducing conditions. This antibody also cross reacts with SOCS-3 from murine cell lines.
SOCS-3 specifically acts as a negative feedback regulator for the STAT3 pathways, by directly binding to the Tyr-759 motif of the IL-6 signal-transducing receptor subunit gp130, thereby preventing the IL-6 activation of STAT3.
The expression of SOCS-3 is inducible by numerous factors, including IL-6, IL-10, toll-like receptor (TLR) agonists and interferon (IFN) gamma, making this protein the focus of research into inflammatory diseases such as arthritis, colitis and Crohn’s disease (Elliott and Johnstone 2004). SOCS-3 expression has also been shown to affect IFN alpha sensitivity in tumor studies (Brender et al. 2001).
Notch signaling plays a critical role in the determination of M1 versus M2 macrophage polarization. RBPJ–mediated notch signaling regulates this polarization through SOCS-3 (Narayana and Balaji 2008, Schroeder and Just 2000).
Mouse anti Human SOCS-3 antibody recognizes human SOCS-3 as a single band of ~25 kDa in MCF7 cell lysates by western blotting under reducing conditions. This antibody also cross reacts with SOCS-3 from murine cell lines.
Product Details
- Target Species
- Human
- Western Blotting
- Anti SOCS-3 antibody detects a band of approximately 25 kDa in MCF7 cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Full length SOCS-3 protein
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg MCF7 lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Guarantee
- As supplied, 12 months from date of despatch.
- Guarantee
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- O14543 Related reagents
- Entrez Gene
- SOCS3 Related reagents
- GO Terms
- GO:0005829 cytosol
- GO:0004860 protein kinase inhibitor activity
- GO:0006916 anti-apoptosis
- GO:0007259 JAK-STAT cascade
- GO:0040008 regulation of growth
- GO:0060333 interferon-gamma-mediated signaling pathway
- GO:0060334 regulation of interferon-gamma-mediated signaling pathway
- GO:0060337 type I interferon-mediated signaling pathway
- GO:0060338 regulation of type I interferon-mediated signaling pathway
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of SOCS-3 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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