SOCS-3 antibody | SO1

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Mouse anti Human SOCS-3

Product Type
Monoclonal Antibody
Clone
SO1
Isotype
IgG2b
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2382 E WB 0.1 mg
Mouse anti Human SOCS-3 antibody, clone SO1 recognizes the human suppressor of cytokine signaling 3 protein, also known as SOCS-3, cytokine-inducible SH2 protein 3 or STAT-induced STAT inhibitor 3. SOCS-3 is a 225 amino acid, ~30 kDa member of the suppressor of cytokine signaling family (Starr et al. 1997) containing a single SH2 and a single SOCS box domain. SOCS-3 acts as a negative regulator of Janus Kinase (JAK) and signal transducer and activators of transcription (STAT) signaling pathways.

SOCS-3 specifically acts as a negative feedback regulator for the STAT3 pathways, by directly binding to the Tyr-759 motif of the IL-6 signal-transducing receptor subunit gp130, thereby preventing the IL-6 activation of STAT3. The expression of SOCS-3 is inducible by numerous factors, including IL-6, IL-10, Toll-like receptor (TLR) agonists and IFN gamma, making this protein the focus of research into inflammatory diseases, such as Arthritis, Colitis, and Crohn’s Disease (Elliott and Johnstone 2004). SOCS-3 expression has also been shown to affect IFN alpha sensitivity in tumour studies (Brender et al.2001).

Notch signaling plays a critical role in the determination of M1 versus M2 macrophage polarization, and that RBPJ–mediated Notch signaling, regulates this polarization through SOCS-3 (Narayana and Balaji 2008, Schroeder and Just 2000).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Full - length SOCS3 protein.
Approx. Protein Concentrations
Ig concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Shelf Life
18 months from date of despatch.

More Information

UniProt
O14543 Related reagents
Entrez Gene
SOCS3 Related reagents
GO Terms
GO:0005829 cytosol
GO:0004860 protein kinase inhibitor activity
GO:0006916 anti-apoptosis
GO:0007259 JAK-STAT cascade
GO:0040008 regulation of growth
GO:0060333 interferon-gamma-mediated signaling pathway
GO:0060334 regulation of interferon-gamma-mediated signaling pathway
GO:0060337 type I interferon-mediated signaling pathway
GO:0060338 regulation of type I interferon-mediated signaling pathway
Regulatory
For research purposes only

Applications of SOCS-3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Western Blotting 1/100 1/500
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
MCA2382 detects a band of approximately 30kDa in Hela cell lysates.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG2b:FITC HCA038F 0.1 mg F
Human anti Mouse IgG2b:HRP HCA038P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Product Specific References

References for SOCS-3 antibody

  1. De Rosa, V. et al. (2007) A key role of leptin in the control of regulatory T cell proliferation.
    Immunity. 26: 241-55.

Further Reading

  1. Lehmann, U. et al. (2003) SHP2 and SOCS3 contribute to Tyr-759-dependent attenuation of interleukin-6 signaling through gp130.
    J Biol Chem. 278 (1): 661-71.
  2. Suzuki, A. et al. (2001) CIS3/SOCS3/SSI3 plays a negative regulatory role in STAT3 activation and intestinal inflammation.
    J Exp Med. 193 (4): 471-81.
  3. Lang, R. et al. (2003) SOCS3 regulates the plasticity of gp130 signaling.
    Nat Immunol. 4 (6): 546-50.
  4. Sakai, I. et al. (2002) Constitutive expression of SOCS3 confers resistance to IFN-alpha in chronic myelogenous leukemia cells.
    Blood. 100 (8): 2926-31.