SMC1A antibody | AB01/4H4

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Mouse anti SMC1A

SMC1A antibody recognizes structural maintenance of chromosomes protein 1A, also known as SMC protein 1A, SMC-1-alpha or segregation of mitotic chromosomes 1.

SMC1A Antibody with Control Lysate

SMC1A antibody recognizes structural maintenance of chromosomes protein 1A, also known as SMC protein 1A, SMC-1-alpha or segregation of mitotic chromosomes 1.

Product Type
PrecisionAb™ Monoclonal
Clone
AB01/4H4
Isotype
IgG1
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00637 WB 100 µl
VMA00637KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human SMC1A antibody recognizes structural maintenance of chromosomes protein 1A, also known as SMC protein 1A, SMC-1-alpha or segregation of mitotic chromosomes 1.

Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1B or the protein encoded by SMC1A. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. SMC1A, which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation. Mutations in SMC1A result in Cornelia de Lange syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms (provided by RefSeq, Jul 2013).

Mouse anti Human SMC1A antibody detects a band of 155 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Mouse anti SMC1A antibody detects a band of approximately 155 kDa in HEK293 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
E. coli-derived recombinant human SMC1A (aa 837-1233)
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HEK293 lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
Q14683 Related reagents
Entrez Gene
SMC1A Related reagents
GO Terms
GO:0000075 cell cycle checkpoint
GO:0000794 condensed nuclear chromosome
GO:0000236 mitotic prometaphase
GO:0000398 nuclear mRNA splicing, via spliceosome
GO:0000777 condensed chromosome kinetochore
GO:0003682 chromatin binding
GO:0003777 microtubule motor activity
GO:0005524 ATP binding
GO:0005737 cytoplasm
GO:0005654 nucleoplasm
GO:0006281 DNA repair
GO:0007052 mitotic spindle organization
GO:0007064 mitotic sister chromatid cohesion
GO:0007091 mitotic metaphase/anaphase transition
GO:0007126 meiosis
GO:0008280 cohesin core heterodimer
GO:0009314 response to radiation
GO:0032876 negative regulation of DNA endoreduplication
GO:0042770 signal transduction in response to DNA damage
GO:0046982 protein heterodimerization activity
GO:0051301 cell division
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of SMC1A antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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