SMC1A antibody | AB01/4H4
Mouse anti SMC1A
SMC1A antibody recognizes structural maintenance of chromosomes protein 1A, also known as SMC protein 1A, SMC-1-alpha or segregation of mitotic chromosomes 1.
SMC1A Antibody with Control Lysate
SMC1A antibody recognizes structural maintenance of chromosomes protein 1A, also known as SMC protein 1A, SMC-1-alpha or segregation of mitotic chromosomes 1.
- Product Type
- PrecisionAb™ Monoclonal
- Clone
- AB01/4H4
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00637 | WB | 100 µl | ||||
| VMA00637KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human SMC1A antibody recognizes structural maintenance of chromosomes protein 1A, also known as SMC protein 1A, SMC-1-alpha or segregation of mitotic chromosomes 1.
Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1B or the protein encoded by SMC1A. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. SMC1A, which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation. Mutations in SMC1A result in Cornelia de Lange syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms (provided by RefSeq, Jul 2013).
Mouse anti Human SMC1A antibody detects a band of 155 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1B or the protein encoded by SMC1A. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. SMC1A, which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation. Mutations in SMC1A result in Cornelia de Lange syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms (provided by RefSeq, Jul 2013).
Mouse anti Human SMC1A antibody detects a band of 155 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti SMC1A antibody detects a band of approximately 155 kDa in HEK293 cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- E. coli-derived recombinant human SMC1A (aa 837-1233)
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HEK293 lysate lyophilized in RIPA buffer
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- Q14683 Related reagents
- Entrez Gene
- SMC1A Related reagents
- GO Terms
- GO:0000075 cell cycle checkpoint
- GO:0000794 condensed nuclear chromosome
- GO:0000236 mitotic prometaphase
- GO:0000398 nuclear mRNA splicing, via spliceosome
- GO:0000777 condensed chromosome kinetochore
- GO:0003682 chromatin binding
- GO:0003777 microtubule motor activity
- GO:0005524 ATP binding
- GO:0005737 cytoplasm
- GO:0005654 nucleoplasm
- GO:0006281 DNA repair
- GO:0007052 mitotic spindle organization
- GO:0007064 mitotic sister chromatid cohesion
- GO:0007091 mitotic metaphase/anaphase transition
- GO:0007126 meiosis
- GO:0008280 cohesin core heterodimer
- GO:0009314 response to radiation
- GO:0032876 negative regulation of DNA endoreduplication
- GO:0042770 signal transduction in response to DNA damage
- GO:0046982 protein heterodimerization activity
- GO:0051301 cell division
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of SMC1A antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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