Mouse anti SMC1A antibody
recognizes structural maintenance of chromosomes protein 1A (SMC1A), also known as SMC-1-alpha. SMC1A is a member of the SMC superfamily and a component of the cohesin complex (Michaelis, C. et al. 1997
). SMC1A also plays a role in DNA repair by acting as an effector in the ATM/NBS1 checkpoint pathway (Yazdi, P.T. et al. 2002
). SMC1A has been implicated in the development of different cancer types. It has been reported to promote colorectal cancer metastasis by recruiting tumor-associated-fibroblasts (Zhou, P. et al. 2017
) and to stimulate growth and migration of prostate cancer (Pan, X.W. et al. 2016
). In human hepatocellular carcinoma, phosphorylated SMC1A is highly over-expressed and associated with patient survival rates (Zhang, Y. et al. 2018
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
- Recombinant human SMC1A (C-terminus) protein fragments expressed in E. coli.
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
cell cycle checkpoint
condensed nuclear chromosome
nuclear mRNA splicing, via spliceosome
condensed chromosome kinetochore
microtubule motor activity
mitotic spindle organization
mitotic sister chromatid cohesion
mitotic metaphase/anaphase transition
cohesin core heterodimer
response to radiation
negative regulation of DNA endoreduplication
signal transduction in response to DNA damage
protein heterodimerization activity
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of SMC1A antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Mouse anti SMC1A antibody recognises a band of approximately 143 kDa in MOLT4, U87 and HeLa cell lysates
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Secondary Antibodies Available