SMC1A antibody | 4C5-C8-A11

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Mouse anti SMC1A

Product Type
Monoclonal Antibody
Clone
4C5-C8-A11
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA6108 WB 50 µg
Mouse anti SMC1A antibody recognizes structural maintenance of chromosomes protein 1A (SMC1A), also known as SMC-1-alpha. SMC1A is a member of the SMC superfamily and a component of the cohesin complex (Michaelis, C. et al. 1997). SMC1A also plays a role in DNA repair by acting as an effector in the ATM/NBS1 checkpoint pathway (Yazdi, P.T. et al. 2002). SMC1A has been implicated in the development of different cancer types. It has been reported to promote colorectal cancer metastasis by recruiting tumor-associated-fibroblasts (Zhou, P. et al. 2017) and to stimulate growth and migration of prostate cancer (Pan, X.W. et al. 2016). In human hepatocellular carcinoma, phosphorylated SMC1A is highly over-expressed and associated with patient survival rates (Zhang, Y. et al. 2018).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
50% Glycerol
Immunogen
Recombinant human SMC1A (C-terminus) protein fragments expressed in E. coli.

Storage Information

Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
Q14683 Related reagents
Entrez Gene
SMC1A Related reagents
GO Terms
GO:0000075 cell cycle checkpoint
GO:0000794 condensed nuclear chromosome
GO:0000236 mitotic prometaphase
GO:0000398 nuclear mRNA splicing, via spliceosome
GO:0000777 condensed chromosome kinetochore
GO:0003682 chromatin binding
GO:0003777 microtubule motor activity
GO:0005524 ATP binding
GO:0005737 cytoplasm
GO:0005654 nucleoplasm
GO:0006281 DNA repair
GO:0007052 mitotic spindle organization
GO:0007064 mitotic sister chromatid cohesion
GO:0007091 mitotic metaphase/anaphase transition
GO:0007126 meiosis
GO:0008280 cohesin core heterodimer
GO:0009314 response to radiation
GO:0032876 negative regulation of DNA endoreduplication
GO:0042770 signal transduction in response to DNA damage
GO:0046982 protein heterodimerization activity
GO:0051301 cell division
Regulatory
For research purposes only

Applications of SMC1A antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Mouse anti SMC1A antibody recognises a band of approximately 143 kDa in MOLT4, U87 and HeLa cell lysates

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F
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