SMARCC2 antibody | AB02/2E2

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Anti SMARCC2 Antibody, clone AB02/2E2 (PrecisionAb™ Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with SMARCC2 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/5,000, STAR207P) and visualized on the ChemiDoc MP with 102 second exposure. Arrow points to SMARCC2 (molecular weight 181 kDa).

Mouse anti SMARCC2

SMARCC2 antibody recognizes SMRC2, also known as Rsc8, BAF170 or CRACC2.

SMARCC2 Antibody with Control Lysate

SMARCC2 antibody recognizes SMRC2, also known as Rsc8, BAF170 or CRACC2.

Product Type: PrecisionAb™ Monoclonal
Clone: AB02/2E2
Isotype: IgG1
Format: Purified

Product Code	Applications	Pack Size
VMA00625	WB	100 µl
VMA00625KT	WB	2 Westerns
With Lysate		400µg

Summary
Secondary Antibodies

Mouse anti Human SMARCC2 antibody recognizes SMARCC2, also known as Rsc8, BAF170 or CRACC2.

The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and contains a predicted leucine zipper motif typical of many transcription factors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene (provided by RefSeq, Jul 2008).

Mouse anti Human SMARCC2 antibody detects a band of 181 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species

Human

Western Blotting

Mouse anti SMARCC2 antibody detects a band of approximately 181 kDa in HEK293 cell lysates

Species Cross-Reactivity

Target Species	Cross Reactivity
Rat

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Product Form

Purified IgG - liquid

Preparation

Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant

Preparation

20μl Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)

Immunogen

E. coli-derived recombinant human SMARCC2 (aa 724-1214)

Instructions For Use

Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition: 400μg HEK293 lysate lyophilized in RIPA buffer
Lysate Reconstitution: - If using DDT reconstitute the lyophilized lysate with 190μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles
Storage: Antibody: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Guarantee: As supplied, 12 months from date of despatch
Guarantee: As supplied, 12 months from date of despatch

More Information

UniProt: Q8TAQ2 Related reagents
Entrez Gene: SMARCC2 Related reagents
GO Terms: GO:0005515 protein binding; GO:0000785 chromatin; GO:0003677 DNA binding; GO:0003682 chromatin binding; GO:0003713 transcription coactivator activity; GO:0006333 chromatin assembly or disassembly; GO:0005654 nucleoplasm; GO:0006338 chromatin remodeling; GO:0006357 regulation of transcription from RNA polymerase II promoter; GO:0007399 nervous system development; GO:0016514 SWI/SNF complex; GO:0045892 negative regulation of transcription, DNA-dependent; GO:0045893 positive regulation of transcription, DNA-dependent; GO:0071564 npBAF complex; GO:0071565 nBAF complex
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory: For research purposes only

Applications of SMARCC2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB

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