SMAD4 antibody | RM277

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Rabbit anti SMAD4

Product Type
Monoclonal Antibody
Clone
RM277
Isotype
IgG
Product Code Applications Pack Size List Price Quantity
MCA6319
Datasheet
P WB
SDS
0.1 ml loader

Rabbit anti Human SMAD4 antibody recognizes mothers against decapentaplegic homolog 4, also known as DPC4, MADH4. SMAD4 is a tumor suppressor which acts as a shuttling protein between the nucleus and the cytoplasm and mediates the TGF-beta signaling pathway, suppressing epithelial cell growth (Ullah et al. 2018). Overexpression of SMAD4 increases apoptosis and suppresses cell proliferation. Accordingly, loss of SMAD4 is linked to various cancers, including those arising in the pancreas, colon, prostate, and bile duct (Chung et al. 2017). Several different SMAD4 isoforms have been found in various cancer types, particularly pancreatic cancer, and can compete against or help cancer cells (Ullah et al. 2018). Over 50% of patients with pancreatic ductal adenocarcinoma (PDAC), one of the most fatal human cancers, involve inactivation of SMAD4 (Ahmed et al. 2017).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography of Protein A from animal origin-free culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
50% Glycerol
Immunogen
A peptide corresponding to the C-terminus of Human SMAD4

Storage Information

Storage
Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use
Guarantee
12 months from date of despatch

More Information

UniProt
Q13485
Entrez Gene
SMAD4
GO Terms
GO:0001666 response to hypoxia
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0005829 cytosol
GO:0030616 transforming growth factor beta receptor, common-partner cytoplasmic mediator activity
GO:0005813 centrosome
GO:0007179 transforming growth factor beta receptor signaling pathway
GO:0007183 SMAD protein complex assembly
GO:0010718 positive regulation of epithelial to mesenchymal transition
GO:0010843 promoter binding
GO:0010862 positive regulation of pathway-restricted SMAD protein phosphorylation
GO:0030308 negative regulation of cell growth
GO:0030509 BMP signaling pathway
GO:0030511 positive regulation of transforming growth factor beta receptor signaling pathway
GO:0032444 activin responsive factor complex
GO:0032909 regulation of transforming growth factor-beta2 production
GO:0042177 negative regulation of protein catabolic process
GO:0042803 protein homodimerization activity
GO:0045892 negative regulation of transcription, DNA-dependent
GO:0060021 palate development
GO:0060391 positive regulation of SMAD protein import into nucleus
GO:0060395 SMAD protein signal transduction
GO:0070411 I-SMAD binding
GO:0070412 R-SMAD binding
Regulatory
For research purposes only

Applications of SMAD4 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1/1000 1/2000
Western Blotting 1/500 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

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