Rabbit anti Human SMAD4 antibody
recognizes mothers against decapentaplegic homolog 4, also known as DPC4, MADH4. SMAD4 is a tumor suppressor which acts as a shuttling protein between the nucleus and the cytoplasm and mediates the TGF-beta signaling pathway, suppressing epithelial cell growth (Ullah et al. 2018
). Overexpression of SMAD4 increases apoptosis and suppresses cell proliferation. Accordingly, loss of SMAD4 is linked to various cancers, including those arising in the pancreas, colon, prostate, and bile duct (Chung et al. 2017
). Several different SMAD4 isoforms have been found in various cancer types, particularly pancreatic cancer, and can compete against or help cancer cells (Ullah et al. 2018
). Over 50% of patients with pancreatic ductal adenocarcinoma (PDAC), one of the most fatal human cancers, involve inactivation of SMAD4 (Ahmed et al. 2017
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of SMAD4 antibody
|Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.