SMAD4 antibody | RM277
Rabbit anti SMAD4
- Product Type
- Monoclonal Antibody
- Clone
- RM277
- Isotype
- IgG
- Specificity
- SMAD4
Antibody Quality and Performance Guarantee
| Rabbit anti Human SMAD4 antibody recognizes mothers against decapentaplegic homolog 4, also known as DPC4, MADH4. SMAD4 is a tumor suppressor which acts as a shuttling protein between the nucleus and the cytoplasm and mediates the TGF-beta signaling pathway, suppressing epithelial cell growth (Ullah et al. 2018). Overexpression of SMAD4 increases apoptosis and suppresses cell proliferation. Accordingly, loss of SMAD4 is linked to various cancers, including those arising in the pancreas, colon, prostate, and bile duct (Chung et al. 2017). Several different SMAD4 isoforms have been found in various cancer types, particularly pancreatic cancer, and can compete against or help cancer cells (Ullah et al. 2018). Over 50% of patients with pancreatic ductal adenocarcinoma (PDAC), one of the most fatal human cancers, involve inactivation of SMAD4 (Ahmed et al. 2017). |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography of Protein A from animal origin-free culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- A peptide corresponding to the C-terminus of Human SMAD4
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin |  |
1/1000 | 1/2000 |
| Western Blotting | |
1/500 | 1/1000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Description | Goat anti Rabbit IgG (Fc):Biotin | ||||||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| Description | Goat anti Rabbit IgG (Fc):FITC | ||||||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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| Description | Goat anti Rabbit IgG (Fc):HRP | ||||||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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| List Price | Your Price | ||||||
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| Description | Goat anti Rabbit IgG (H/L):HRP | ||||||
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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| List Price | Your Price | ||||||
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| Description | Sheep anti Rabbit IgG:RPE | ||||||
- UniProt
- Q13485
- Entrez Gene
- SMAD4
- GO Terms
- GO:0001666 response to hypoxia
- GO:0003700 sequence-specific DNA binding transcription factor activity
- GO:0005829 cytosol
- GO:0030616 transforming growth factor beta receptor, common-partner cytoplasmic mediator activity
- GO:0005813 centrosome
- GO:0007179 transforming growth factor beta receptor signaling pathway
- GO:0007183 SMAD protein complex assembly
- GO:0010718 positive regulation of epithelial to mesenchymal transition
- GO:0010843 promoter binding
- View More GO Terms
- GO:0010862 positive regulation of pathway-restricted SMAD protein phosphorylation
- GO:0030308 negative regulation of cell growth
- GO:0030509 BMP signaling pathway
- GO:0030511 positive regulation of transforming growth factor beta receptor signaling pathway
- GO:0032444 activin responsive factor complex
- GO:0032909 regulation of transforming growth factor-beta2 production
- GO:0042177 negative regulation of protein catabolic process
- GO:0042803 protein homodimerization activity
- GO:0045892 negative regulation of transcription, DNA-dependent
- GO:0060021 palate development
- GO:0060391 positive regulation of SMAD protein import into nucleus
- GO:0060395 SMAD protein signal transduction
- GO:0070411 I-SMAD binding
- GO:0070412 R-SMAD binding
MCA6319
155789
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