SIRT6 antibody | EF01/2A11

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Mouse anti SIRT6

Product Type
PrecisionAb Monoclonal
Clone
EF01/2A11
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00961
Datasheet
WB
SDS
100 µl loader

Mouse anti SIRT6 antibody recognizes NAD-dependent protein deacetylase sirtuin-6, also known as regulatory protein SIR2 homolog 6. SIRT6 is a NAD+-dependent deacetylase which belongs to the sirtuin family. As a histone deacetylase, SIRT6 targets specific sites on histone H3 and regulates chromatin compaction, transcriptional repression and DNA damage responses. The protein has multiple targets, and through its deacetylase activity, plays roles in chromatin signaling and genome maintenance (Tasselli et al. 2016). SIRT6 can be found in the nucleus and in the cytoplasm, and localizes to cytoplasmic stress granules in response to stress (D'Onofrio et al. 2018). SIRT6 protects against aging-associated pathologies such as metabolic disease and cancer (Tasselli et al. 2016). SIRT6 appears to have a protective role against obesity and diabetes, with deficiency of SIRT6 leading to liver steatosis, diet-induced obesity and diabetes (Kuang et al. 2018).

Product Details

Target Species
Human
Western Blotting
Mouse anti SIRT6 detects a band of approximately 40 kDa in Jurkat cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-353 of human SIRT6
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
Q8N6T7
Entrez Gene
SIRT6
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of SIRT6 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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