SIRT6 antibody | EF01/2A11
Mouse anti SIRT6 antibody recognizes NAD-dependent protein deacetylase sirtuin-6, also known as regulatory protein SIR2 homolog 6. SIRT6 is a NAD+-dependent deacetylase which belongs to the sirtuin family. As a histone deacetylase, SIRT6 targets specific sites on histone H3 and regulates chromatin compaction, transcriptional repression and DNA damage responses. The protein has multiple targets, and through its deacetylase activity, plays roles in chromatin signaling and genome maintenance (Tasselli et al. 2016). SIRT6 can be found in the nucleus and in the cytoplasm, and localizes to cytoplasmic stress granules in response to stress (D'Onofrio et al. 2018). SIRT6 protects against aging-associated pathologies such as metabolic disease and cancer (Tasselli et al. 2016). SIRT6 appears to have a protective role against obesity and diabetes, with deficiency of SIRT6 leading to liver steatosis, diet-induced obesity and diabetes (Kuang et al. 2018).
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti SIRT6 detects a band of approximately 40 kDa in Jurkat cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - Liquid
- Preparation
- Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
- Immunogen
- E. coli-derived recombinant protein of amino acids 1-353 of human SIRT6
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q8N6T7
- Entrez Gene
- SIRT6
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of SIRT6 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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