SIRT6 antibody | EF01/2A11

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Anti SIRT6 Antibody, clone EF01/2A11 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti SIRT6 Antibody, clone EF01/2A11 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with SIRT6 antibody (1/1000, **VMA00961**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to SIRT6 (molecular weight 40 kDa)

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Mouse anti SIRT6

Product Type: PrecisionAb Monoclonal
Clone: EF01/2A11
Isotype: IgG1
Format: Purified
Specificity: SIRT6

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VMA00961 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Mouse anti SIRT6 antibody recognizes NAD-dependent protein deacetylase sirtuin-6, also known as regulatory protein SIR2 homolog 6. SIRT6 is a NAD+-dependent deacetylase which belongs to the sirtuin family. As a histone deacetylase, SIRT6 targets specific sites on histone H3 and regulates chromatin compaction, transcriptional repression and DNA damage responses. The protein has multiple targets, and through its deacetylase activity, plays roles in chromatin signaling and genome maintenance (Tasselli et al. 2016). SIRT6 can be found in the nucleus and in the cytoplasm, and localizes to cytoplasmic stress granules in response to stress (D'Onofrio et al. 2018). SIRT6 protects against aging-associated pathologies such as metabolic disease and cancer (Tasselli et al. 2016). SIRT6 appears to have a protective role against obesity and diabetes, with deficiency of SIRT6 leading to liver steatosis, diet-induced obesity and diabetes (Kuang et al. 2018).

Target Species: Human
Western Blotting: Mouse anti SIRT6 detects a band of approximately 40 kDa in Jurkat cell lysates
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - Liquid
Preparation: Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide
Immunogen: E. coli-derived recombinant protein of amino acids 1-353 of human SIRT6
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml
Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories

Target Species	Cross Reactivity
Mouse
Rat

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.