SIRT2 antibody | AB01/1D10

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Anti SIRT2 Antibody, clone AB01/1D10 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti SIRT2 Antibody, clone AB01/1D10 (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti SIRT2 Antibody, clone AB01/1D10 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with SIRT2 antibody (1/1000, **VMA00960**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/5000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to SIRT2 (molecular weight 39 and 44 kDa)

Anti SIRT2 Antibody, clone AB01/1D10 (PrecisionAb Monoclonal Antibody) gallery image 2 — Western blot analysis of whole cell lysates probed with SIRT2 antibody (1/500, **VMA00960**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/5000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to SIRT2 (molecular weight 39 and 44 kDa)

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Mouse anti SIRT2

Product Type: PrecisionAb Monoclonal
Clone: AB01/1D10
Isotype: IgG2b
Format: Purified
Specificity: SIRT2

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Product Code Applications Pack Size List Price Your Price Qty

List Price	Your Price
VMA00960 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Mouse anti SIRT2 antibody recognizes NAD-dependent protein deacetylase sirtuin-2, also known as regulatory protein SIR2 homolog 2. SIRT2 is a NAD+-dependent deacetylase which belongs to the sirtuin family and plays a wide range of roles in the nervous system, regulation of mitosis, genome integrity, cell differentiation, infection, inflammation, and in many other processes (Wang et al. 2019). SIRT2 also has diverse roles in metabolism. Consistent with its multiple roles, SIRT2 is expressed in a wide range of tissues, particularly tissues which are metabolically active such as the heart, brain, and adipose tissue (Tang et al. 2017). SIRT2 has been shown to have tumor-promoting activity. Inhibition of SIRT2 has been found to promote ubiquitination and degradation of c-Myc and has broad anticancer effects across a range of human cancer cell lines (Jing et al. 2016).

Target Species: Human
Western Blotting: Mouse anti SIRT2 detects a band of approximately 39 and 44 kDa in HEK293 cell lysates
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - Liquid
Preparation: Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide
Immunogen: E. coli-derived recombinant protein of amino acids 1-389 of human SIRT2
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml
Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories

Target Species	Cross Reactivity
Mouse
Rat

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/500	1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: Q8IXJ6
Entrez Gene: SIRT2
GO Terms: GO:0000183 chromatin silencing at rDNA; GO:0005737 cytoplasm; GO:0005677 chromatin silencing complex; GO:0005874 microtubule; GO:0006348 chromatin silencing at telomere; GO:0006471 protein ADP-ribosylation; GO:0007067 mitosis; GO:0007096 regulation of exit from mitosis; GO:0008134 transcription factor binding; View More GO Terms; GO:0008270 zinc ion binding; GO:0016575 histone deacetylation; GO:0017136 NAD-dependent histone deacetylase activity; GO:0035035 histone acetyltransferase binding; GO:0042325 regulation of phosphorylation; GO:0042826 histone deacetylase binding; GO:0042903 tubulin deacetylase activity; GO:0043130 ubiquitin binding; GO:0045843 negative regulation of striated muscle tissue development; GO:0051301 cell division; GO:0051775 response to redox state; GO:0070403 NAD+ binding