SIRT2 antibody | AB01/1D10
Mouse anti SIRT2 antibody recognizes NAD-dependent protein deacetylase sirtuin-2, also known as regulatory protein SIR2 homolog 2. SIRT2 is a NAD+-dependent deacetylase which belongs to the sirtuin family and plays a wide range of roles in the nervous system, regulation of mitosis, genome integrity, cell differentiation, infection, inflammation, and in many other processes (Wang et al. 2019). SIRT2 also has diverse roles in metabolism. Consistent with its multiple roles, SIRT2 is expressed in a wide range of tissues, particularly tissues which are metabolically active such as the heart, brain, and adipose tissue (Tang et al. 2017). SIRT2 has been shown to have tumor-promoting activity. Inhibition of SIRT2 has been found to promote ubiquitination and degradation of c-Myc and has broad anticancer effects across a range of human cancer cell lines (Jing et al. 2016).
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti SIRT2 detects a band of approximately 39 and 44 kDa in HEK293 cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - Liquid
- Preparation
- Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
- Immunogen
- E. coli-derived recombinant protein of amino acids 1-389 of human SIRT2
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q8IXJ6
- Entrez Gene
- SIRT2
- GO Terms
- GO:0000183 chromatin silencing at rDNA
- GO:0005737 cytoplasm
- GO:0005677 chromatin silencing complex
- GO:0005874 microtubule
- GO:0006348 chromatin silencing at telomere
- GO:0006471 protein ADP-ribosylation
- GO:0007067 mitosis
- GO:0007096 regulation of exit from mitosis
- GO:0008134 transcription factor binding
- GO:0008270 zinc ion binding
- GO:0016575 histone deacetylation
- GO:0017136 NAD-dependent histone deacetylase activity
- GO:0035035 histone acetyltransferase binding
- GO:0042325 regulation of phosphorylation
- GO:0042826 histone deacetylase binding
- GO:0042903 tubulin deacetylase activity
- GO:0043130 ubiquitin binding
- GO:0045843 negative regulation of striated muscle tissue development
- GO:0051301 cell division
- GO:0051775 response to redox state
- GO:0070403 NAD+ binding
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of SIRT2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/500 | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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